Hull S, Benghuzzi H, Tucci M, Hughes J
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:73-8.
Recently, several studies have indicated that the use of Rhesus Monkey Kidney epithelial cells (RMKEC) in culture could provide significant knowledge regarding the alteration or dysfunction of kidney tissues that often resulted into kidney failure. The interrelationship between various steroid hormones, as well as, growth-promoting hormones such as growth hormone (GH) and RMKEC has not been fully investigated. The specific objective of this study was to investigate the effects of cortisol (C), testosterone (T), dehydroepiandrosterone (DHEA), estradiol (E), and GH on the proliferation and viability of RMKEC in culture. The cell line was adapted to grow in Morgan, Morton, and Parker's medium 199 (with 1.68 g/L sodium bicarbonate) supplemented with 1% horse serum. A total of 30 tubes were plated with RMKEC and divided into six equal groups. In-groups 1-5, each well (n = 5) were treated with a physiological dose of C, T, DHEA, E, and GH, respectively. At 24, 48, and 96 hours the cells and supernatants were collected and stored for further analysis. The biochemical markers were assessed using lactate dehydrogenase (LDH), catalase, and malinodialdehyde (MDA). Data obtained suggest that: (I) treatment of RMKEC with C and DHEA resulted in an increase in MDA levels compared to the control and other experimental groups, (II) no significant increase was observed in LDH levels in all treated tubes compared to the control group, (III) higher proliferation rate was observed in cells treated with T compared to the control group. However, treatment with C showed suppression to the proliferation rate and no significant difference was observed between DHEA, GH and the control groups. In conclusion this study suggests that steroid hormones regardless of the source of secretion (gonads or adrenals) can influence the functional capacity of RMKEC in culture.
最近，多项研究表明，在培养中使用恒河猴肾上皮细胞（RMKEC）可为了解经常导致肾衰竭的肾组织改变或功能障碍提供重要知识。各种类固醇激素以及生长促进激素（如生长激素（GH））与RMKEC之间的相互关系尚未得到充分研究。本研究的具体目的是研究皮质醇（C）、睾酮（T）、脱氢表雄酮（DHEA）、雌二醇（E）和GH对培养的RMKEC增殖和活力的影响。该细胞系适应于在补充有1%马血清的摩根、莫顿和帕克199培养基（含1.68 g/L碳酸氢钠）中生长。总共接种了30管RMKEC，并将其分成六个相等的组。在第1 - 5组中，每个孔（n = 5）分别用生理剂量的C、T、DHEA、E和GH处理。在24、48和96小时时收集细胞和上清液并储存以供进一步分析。使用乳酸脱氢酶（LDH）、过氧化氢酶和丙二醛（MDA）评估生化标志物。获得的数据表明：（I）与对照组和其他实验组相比，用C和DHEA处理RMKEC导致MDA水平升高，（II）与对照组相比，所有处理管中的LDH水平均未观察到显著升高，（III）与对照组相比，用T处理的细胞观察到更高的增殖率。然而，用C处理显示对增殖率有抑制作用，并且在DHEA、GH与对照组之间未观察到显著差异。总之，本研究表明，无论分泌来源（性腺或肾上腺）如何，类固醇激素均可影响培养的RMKEC的功能能力。
