Vance Stacy Hull, Tucci Michelle, Benghuzzi Hamed
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 2007;43:260-5.
The ability to utilize cell cultures to predict the effects of drugs and other compounds in in vivo models is an invaluable tool. Various studies have investigated the effects of kidney epithelial cells to various agents; however there are few studies that compare an in vitro to an in vivo environment. The specific objective of this study was to investigate the pathophysiological effects of Cortisol (CORT) on the proliferation and viability of Rhesus monkey kidney epithelial cells (RMKEC) in vitro. Twenty-four wells were plated with RMKEC and divided into four equal groups. Cells were treated with 10microL of CORT 5 microg/dl, CORT 20 microg/dl, or CORT 50 microg/d1 at 24, 48, and 72 hours. Epithelial damage was evident 24 after receiving a supraphysiological dose of cort, with increased hydrophic effects and alterations in cellular metabolism. The overall histological and cytological data suggest that test compounds tested in tissue culture provide a correlation as to how they will perform in vivo. Testing in compounds in vitro will allow the investigator to establish the IC50's of the test compounds as well as calculate the number of animals needed to reach statistical power.
利用细胞培养来预测药物和其他化合物在体内模型中的作用的能力是一种非常宝贵的工具。各种研究已经调查了肾上皮细胞对各种药物的反应；然而，很少有研究比较体外和体内环境。本研究的具体目标是在体外研究皮质醇（CORT）对恒河猴肾上皮细胞（RMKEC）增殖和活力的病理生理作用。将RMKEC接种到24孔板中，并分成四个相等的组。在24、48和72小时时，用10微升5微克/分升、20微克/分升或50微克/分升的CORT处理细胞。在接受超生理剂量的皮质醇后24小时，上皮损伤明显，出现疏水作用增加和细胞代谢改变。总体组织学和细胞学数据表明，在组织培养中测试的受试化合物与其在体内的表现具有相关性。在体外测试化合物将使研究人员能够确定受试化合物的半数抑制浓度（IC50），并计算达到统计学效力所需的动物数量。
