Evgen'eva T P
Sov J Dev Biol. 1975 Jan;5(1):66-9.
Minute fragments of the notochord of Balanoglossus sp. were cultivated in diffusion chambers implanted into the arm cavities of starfish, Asterias rubens. The surface properties of the cells migrating from these fragments and the intercellular contacts between them were examined in a scanning electron microscope type "Stereoscan." The two types of cell constituting the initial notochordal transplantate migrated already on the 2nd day of cultivation; these were rather large (10-12 mu) round cells with characteristic outgrowths, which grouped into colonies already on the 3rd-5th day of growth in culture, and small cells (5-7 mu) of elongated shape that at early cultivation stages were found scattered in a disorderly manner over the filter. At later cultivation stages (days 10-12), the small cells migrated toward the colonies of round notochordal cells and penetrated inside them. As a result, large colonies (counting 100 or more cells) developed in the cultures, their arrangement resembling that of the donor animal's notochord.
将柱头虫属（Balanoglossus sp.）脊索的微小片段培养在植入到红海盘车（Asterias rubens）腕腔中的扩散小室中。用一台“Stereoscan”型扫描电子显微镜检查了从这些片段迁移出来的细胞的表面特性以及它们之间的细胞间接触情况。构成最初脊索移植物的两种类型的细胞在培养的第2天就开始迁移；这些是相当大的（10 - 12微米）圆形细胞，带有特征性的突出物，在培养的第3 - 5天就聚集成菌落，还有细长形的小细胞（5 - 7微米），在培养早期发现它们无序地散布在滤膜上。在培养后期（第10 - 12天），小细胞向圆形脊索细胞的菌落迁移并穿透到它们内部。结果，培养物中形成了大的菌落（包含100个或更多细胞），它们的排列类似于供体动物的脊索。
