Nozdrachev A D, Skopichev V G, Osipova N S
Fiziol Zh SSSR Im I M Sechenova. 1983 Dec;69(12):1581-8.
A culture of the neurons of metasympathetic system's enteral portion of 3-5-day old rats was obtained and fragments of the mienteral plexus were implanted to adult rats-recipients. A technique of cultivation was developed for in vivo microscopy of culture's neurons. Migration of neuroblasts from the deplantate area was revealed as well as the specific features of neurons differentiation. The neurons were shown to acquire characteristic morphological features corresponding to Dogel's I and II types of neurons; growth of neurites was shown to aid to formation of neuropil and microglia. Histochemical analysis revealed a product of response to cholinesterase which is the index of formation of the acetylcholine hydrolysis system in the culture's I type neurons. The technique of electron-microscopic scanning enables to watch in detail the process of formation of interneuronal connections and formation of a synapse.
获取了3 - 5日龄大鼠交感性神经系统肠内部分神经元的培养物，并将肠肌丛碎片植入成年大鼠受体。开发了一种用于培养神经元体内显微镜观察的培养技术。观察到神经母细胞从移植区的迁移以及神经元分化的特定特征。这些神经元呈现出与多吉尔I型和II型神经元相对应的特征性形态特征；神经突的生长有助于神经毡和小胶质细胞的形成。组织化学分析显示了胆碱酯酶反应产物，这是培养物中I型神经元乙酰胆碱水解系统形成的指标。电子显微镜扫描技术能够详细观察神经元间连接的形成过程和突触的形成。
