Lee K W, Jeon H, Park J Y
Department of Clinical Pathology, College of Medicine, Hallym University, Seoul, Korea.
Tissue Antigens. 2000 Nov;56(5):428-35. doi: 10.1034/j.1399-0039.2000.560505.x.
Alleles in the HLA-B15 group encode molecules belonging to several serologic subgroups, B15 (B62, B63, B75, B76, B77) and B70 (B71, B72), representing many of the most problematic types to assign in routine clinical typing laboratories due to their serologic cross-reactivity resulting from structural similarity. More than 25% of Koreans express HLA-B molecules encoded by the HLA-B15 alleles. To further characterize HLA-B15 in this population, B15-specific polymerase chain reaction (PCR) and sequence-specific oligonucleotide probe (SSOP) hybridization analysis using 39 digoxigenin-labeled probes were applied to DNA samples obtained from 237 B15/B70 serologically positive unrelated individuals. Nine B15 alleles were identified. B1501 was the most frequent allele (64.8%) followed by B1511 (14.1%), B1507 (8.6%), and B1518 (5.5%) comprising more than 90% of B15-positive samples. B62 molecules encoded by 4 of the identified alleles (B1501, B1507, B1525, and B1527) could not be discriminated by serologic reaction patterns. Among the fifteen B15/B70 apparent homozygotes, eight were heterozygotes carrying two different B15 alleles. Several B15 alleles exhibited strong associations with specific Cw, DRB1, and A allelic types (e.g., B1507-Cw3 (22/22); B1507-DRB104 (21/22), B1507-A24 (17/22)). The data obtained in this study confirmed B*15 diversity in the study population and will be useful in hematopoietic stem cell donor searches as well as in determining the supplementary DNA typing strategy for B15/B70-positive samples in this population.
HLA - B15组中的等位基因编码属于几个血清学亚组的分子，即B15（B62、B63、B75、B76、B77）和B70（B71、B72），由于其结构相似导致血清学交叉反应，它们是常规临床分型实验室中最难分型的类型之一。超过25%的韩国人表达由HLA - B15等位基因编码的HLA - B分子。为了进一步表征该人群中的HLA - B15，使用39种地高辛标记探针的B15特异性聚合酶链反应（PCR）和序列特异性寡核苷酸探针（SSOP）杂交分析应用于从237名B15/B70血清学阳性无关个体获得的DNA样本。鉴定出9个B15等位基因。B1501是最常见的等位基因（64.8%），其次是B1511（14.1%）、B1507（8.6%）和B1518（5.5%），它们占B15阳性样本的90%以上。由4个已鉴定等位基因（B1501、B1507、B1525和B1527）编码的B62分子无法通过血清学反应模式进行区分。在15个B15/B70明显纯合子中，有8个是携带两种不同B15等位基因的杂合子。几个B15等位基因与特定的Cw、DRB1和A等位基因类型表现出强关联（例如，B1507 - Cw3（22/22）；B1507 - DRB104（21/22），B1507 - A24（17/22））。本研究获得的数据证实了该研究人群中B*15的多样性，这将有助于造血干细胞供体搜索以及确定该人群中B15/B70阳性样本的补充DNA分型策略。
