Lin L, Tokunaga K, Tanaka H, Nakajima F, Imanishi T, Kashiwase K, Bannai M, Mizuno S, Akaza T, Tadokoro K, Shibata Y, Juji T
Department of Research, Japanese Red Cross Central Blood Center, Tokyo, Japan.
Tissue Antigens. 1996 Apr;47(4):265-74. doi: 10.1111/j.1399-0039.1996.tb02553.x.
In order to further clarify the diversity of the HLA-B15 antigens and the correspondence of serological types with alleles in Asians, we screened various B15 serological splits by means of a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method. Subsequently, the genes encoding various B15 variants were sequenced. Two novel alleles, B1528 and B1529, were identified: the nucleotide sequence of the former contained a single-base substitution at position 263 in exon 2 as compared to that of the B1501 allele, which results in an amino acid change at position 64 in the alpha 1 domain, and the nucleotide sequence of the latter differs from that of B1518 by a single-base substitution at position 272 of exon 2 which results in an amino acid change at position 67 of the alpha 1 domain. One new allele, B1521, described recently in Australian Aborigines was also identified in Asians in the present study. Moreover, the results of sequencing demonstrated that Asian HLA-B62, B70, and B77 antigens are encoded by B1501, B1518, and B1513, respectively. Two splits of B75 antigens, B75V (TS-1) and B15N, which have been proposed to exist in the Japanese population were encoded by B1511 and B1502, respectively. Most of the B15 alleles detected in the present study showed positive associations with other locus antigens. Especially, B1502 was strongly associated with Cw8, while B1521 was strongly associated with A34 and Cw6.
为了进一步阐明亚洲人中HLA - B15抗原的多样性以及血清学类型与等位基因的对应关系，我们采用聚合酶链反应 - 单链构象多态性（PCR - SSCP）方法筛选了各种B15血清学分型。随后，对编码各种B15变体的基因进行了测序。鉴定出两个新的等位基因，B1528和B1529：与B1501等位基因相比，前者的核苷酸序列在第2外显子的第263位有一个单碱基替换，这导致α1结构域第64位的氨基酸发生变化；后者的核苷酸序列与B1518的不同之处在于第2外显子的第272位有一个单碱基替换，这导致α1结构域第67位的氨基酸发生变化。在本研究中，在亚洲人中还鉴定出了最近在澳大利亚原住民中描述的一个新等位基因B1521。此外，测序结果表明，亚洲人HLA - B62、B70和B77抗原分别由B1501、B1518和B1513编码。在日本人群中曾提出存在的B75抗原的两个亚型B75V（TS - 1）和B15N，分别由B1511和B1502编码。在本研究中检测到的大多数B15等位基因与其他位点抗原呈正相关。特别是，B1502与Cw8强烈相关，而B1521与A34和Cw6强烈相关。
