Blanc D S, Bañuls A L, Hauser P M, Moreillon P, Francioli P, Tibayrenc M
Division autonome de médecine préventive hospitalière, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Microb Drug Resist. 2000 Fall;6(3):231-8. doi: 10.1089/mdr.2000.6.231.
We have compared the phylogenetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains from Switzerland and their phylogenetic relationships with European epidemic clones, using multiprimer random amplification polymorphic DNA (RAPD). Strains included 24 European epidemic clones (59 strains), 66 sporadic strains isolated in Switzerland in 1996-1997, and 15 reference strains of five other Staphylococcus species. Similarity and clustering analysis with the Jaccard's coefficient showed that the maximum genetic distance between MRSA strains was 0.43, whereas the minimum genetic distance between the six Staphylococcus species was 0.97, indicating that the method permits phylogenetic hierarchization. The 24 MRSA clones reported to be epidemic in European countries during the 1990s were distributed into seven different genetic clusters with a maximum distance of 0.29 among them. This clustering pattern was confirmed by the analysis of a subset of MRSA strains by multilocus enzyme electrophoresis at 12 loci. Most of the sporadic Swiss strains were distributed into these seven different genetic clusters, together with the epidemic MRSA clones. This suggests that there is no phylogenetic cluster specific to epidemic clones of MRSA.
我们使用多引物随机扩增多态性DNA（RAPD）技术，比较了来自瑞士的耐甲氧西林金黄色葡萄球菌（MRSA）菌株的系统发育多样性，以及它们与欧洲流行克隆的系统发育关系。菌株包括24个欧洲流行克隆（59株）、1996 - 1997年在瑞士分离的66株散发菌株，以及其他5种葡萄球菌的15株参考菌株。用杰卡德系数进行相似性和聚类分析表明，MRSA菌株之间的最大遗传距离为0.43，而6种葡萄球菌之间的最小遗传距离为0.97，这表明该方法能够进行系统发育层次划分。20世纪90年代在欧洲国家报告的24个MRSA克隆被分为7个不同的遗传簇，它们之间的最大距离为0.29。通过对12个位点的多位点酶电泳分析MRSA菌株的一个子集，证实了这种聚类模式。大多数瑞士散发菌株与流行的MRSA克隆一起分布在这7个不同的遗传簇中。这表明不存在特定于MRSA流行克隆的系统发育簇。
