Date I, Shingo T, Yoshida H, Fujiwara K, Kobayashi K, Ohmoto T
Department of Neurological Surgery, Okayama University Medical School, Japan.
Cell Transplant. 2000 Sep-Oct;9(5):705-9. doi: 10.1177/096368970000900519.
The transplantation of encapsulated dopamine-secreting cells into the striatum represents one potential means of treating Parkinson's disease. The present study investigated the ability of encapsulated PC12 cells, which are derived from rat pheochromocytoma, to supply L-dopa and dopamine into the primate brain in the long term and to effect functional improvement in the animals. Following polymer encapsulation, PC12 cells were transplanted into the striatum of hemiparkinsonian monkeys. The secretion of L-dopa and dopamine from the encapsulated cells, the morphology of these cells, the histology of the host striatum surrounding the capsule, and functional changes in the host animals were examined 1, 6, and 12 months after transplantation. Analysis of retrieved capsules revealed that the PC12 cells survived and continued to release L-dopa and dopamine even 12 months after transplantation. The histological response of the host brain surrounding the capsules was minimal and there were no signs of immunological rejection or tumor formation. The physical condition of the host animals was good for 12 months, and hematologic and cerebrospinal fluid analysis revealed that no animals suffered from infection or immunological reaction. These PC12 cell-grafted monkeys showed improvements in hand movements after transplantation, effects that lasted for at least 12 months. These results further support the potential use of this approach for the treatment of Parkinson's disease.
将包裹分泌多巴胺细胞移植到纹状体是治疗帕金森病的一种潜在方法。本研究调查了源自大鼠嗜铬细胞瘤的包裹PC12细胞长期向灵长类动物脑内供应左旋多巴和多巴胺以及改善动物功能的能力。经聚合物包裹后，将PC12细胞移植到偏侧帕金森病猴的纹状体中。在移植后1个月、6个月和12个月，检测包裹细胞中左旋多巴和多巴胺的分泌、这些细胞的形态、包裹物周围宿主纹状体的组织学以及宿主动物的功能变化。对回收的包裹物分析显示，即使在移植后12个月，PC12细胞仍存活并继续释放左旋多巴和多巴胺。包裹物周围宿主脑的组织学反应轻微，没有免疫排斥或肿瘤形成的迹象。宿主动物的身体状况在12个月内良好，血液学和脑脊液分析显示没有动物发生感染或免疫反应。这些移植了PC12细胞的猴子在移植后手部运动有所改善，这种效果持续了至少12个月。这些结果进一步支持了这种方法在帕金森病治疗中的潜在应用。
