Franchini A, Ottaviani E
Department of Animal Biology, University of Modena and Reggio Emilia, Modeno, Italy.
Tissue Cell. 2000 Aug;32(4):312-21. doi: 10.1054/tice.2000.0118.
Various cellular and humoral activities of the wound repair process and the effects of PDGF-AB and TGF-beta1 on tissue repair mechanisms in the mollusc Limax maximus are studied by histological and immunocytochemical procedures. Histological examination at different times after the wound production demonstrates that tissue repair is the result of successive and related activities distinguishable by different morphological pictures. In the first hours, an infiltration phase is activated 24 h after the incision, hemocytes stratify at wound margins and actively phagocitize cell debris and damaged tissue in the surrounding area. Moreover, the cells are immunoreactive to anti-IL-1alpha, IL-8 and TNF-alpha antibodies. After 24-72 h, granulation tissue rich in small blood lacunae is formed and the provisional matrix is synthesized and deposited on the base of the new tissue. In histological sections 72 h after injury, the incision is filled with granulation tissue, and at the wound base, a layer of fibrous connective tissue is formed. Hemocytes present in the newly formed blood lacunae and fibroblasts are involved in the synthesis and deposit of extracellular matrix components, i.e. fibronectin, reticular and collagen fibres. Ninety-six h after wound production, the repair process continues and the granulation tissue is more developed. At 192 h, re-epithelialization begins, and this is more evident in the histological sections after 14 days. Hemocytes are immunoreactive to the cytokines at all the times examined. Exogenous administration of PDGF-AB and TGF-beta1 stimulates the tissue healing process through a general acceleration of the activities involved. A larger closing area of clumped hemocytes and a smaller damaged tissue area are observed 24 h after treatment of the wound. At 72 h, the granulation tissue is more developed and more extracellular matrix components are deposited than in the control incision. A larger number of cells express cytokine-like molecules, and re-epithelialization of the wound is accelerated, as 14 days after growth factor treatments almost all the wound area is covered by a layer of cubic epithelial cells, and the alcianophilic cell coat is restored. No differences in the responses of the two growth factors are observed.
通过组织学和免疫细胞化学方法，研究了蛞蝓（Limax maximus）伤口修复过程中的各种细胞和体液活动，以及血小板衍生生长因子AB（PDGF-AB）和转化生长因子β1（TGF-β1）对组织修复机制的影响。伤口产生后不同时间的组织学检查表明，组织修复是由不同形态学图像区分的连续且相关活动的结果。在最初的几个小时内，损伤后24小时激活浸润阶段，血细胞在伤口边缘分层，并积极吞噬周围区域的细胞碎片和受损组织。此外，这些细胞对抗白细胞介素-1α（IL-1α）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）抗体具有免疫反应性。24至72小时后，形成富含小血腔的肉芽组织，并在新组织的基础上合成和沉积临时基质。在损伤后72小时的组织学切片中，切口充满肉芽组织，在伤口底部形成一层纤维结缔组织。新形成的血腔中的血细胞和成纤维细胞参与细胞外基质成分（即纤连蛋白、网状纤维和胶原纤维）的合成和沉积。伤口产生96小时后，修复过程继续，肉芽组织更加发达。在192小时时，重新上皮化开始，在14天后的组织学切片中更为明显。在所有检查的时间点，血细胞对细胞因子都具有免疫反应性。外源性给予PDGF-AB和TGF-β1通过全面加速相关活动来刺激组织愈合过程。伤口处理24小时后，观察到聚集血细胞的封闭面积更大，受损组织面积更小。在72小时时，肉芽组织比对照切口更发达，沉积的细胞外基质成分更多。大量细胞表达细胞因子样分子，伤口的重新上皮化加速，因为生长因子处理14天后，几乎所有伤口区域都被一层立方上皮细胞覆盖，亲阿尔辛蓝细胞被膜恢复。未观察到两种生长因子反应的差异。
