Derby E G, Reddy V, Nelson E L, Kopp W C, Baseler M W, Dawson J R, Malyguine A M
NCI-Frederick Cancer Research and Development Center, SAIC-Frederick, Frederick, MD 21702-1201, USA.
Cytokine. 2001 Jan 21;13(2):85-90. doi: 10.1006/cyto.2000.0804.
The use of an IFN-gamma ELISPOT assay to evaluate cellular immune responses has gained increasing popularity, especially as a surrogate measure for cytotoxic T lymphocyte (CTL) responses. We have compared the IFN-gamma ELISPOT assay and the traditional(51)Cr release assay for detection of human natural killer (NK) cell activity. The cell populations used for evaluation of these assays included freshly isolated and IL-2-activated peripheral blood mononuclear cells (PBMC). CD56-positive cells were demonstrated to be the primary source of the IFN-gamma signal when PBMC were evaluated with NK-sensitive targets in the IFN-gamma ELISPOT assay. IFN-gamma ELISPOT and(51)Cr release assays showed excellent correlation suggesting that NK activity can be reliably evaluated with methods other than the traditional(51)Cr release assays.
使用γ干扰素酶联免疫斑点（ELISPOT）检测法评估细胞免疫反应越来越受到欢迎，尤其是作为细胞毒性T淋巴细胞（CTL）反应的替代指标。我们比较了γ干扰素ELISPOT检测法和传统的铬（51）释放检测法用于检测人类自然杀伤（NK）细胞活性。用于评估这些检测法的细胞群体包括新鲜分离的和经白细胞介素-2激活的外周血单个核细胞（PBMC）。当在γ干扰素ELISPOT检测法中用对NK敏感的靶标评估PBMC时，CD56阳性细胞被证明是γ干扰素信号的主要来源。γ干扰素ELISPOT检测法和铬（51）释放检测法显示出极好的相关性，这表明除了传统的铬（51）释放检测法外，还可以用其他方法可靠地评估NK活性。
