Sakamoto T, Tanaka T, Umesaki N, Ogita S
Department of Obstetrics and Gynecology, Osaka City University Medical School, Osaka, Japan.
Horm Res. 2000;53(6):294-9. doi: 10.1159/000053186.
The effects of epidermal growth factor (EGF) on human endometrial stromal cells have not been characterized well, although production of EGF in endometrial epithelial and stromal cells and expression of EGF receptors in endometrial stromal cells have been reported. We investigated the effects of EGF on endometrial cell viability, 8-Br-cAMP-induced stromal decidualization, and prolactin secretion from decidualized endometrial stromal cells using an in vitro decidualization activity assay of human endometrial stromal cells. EGF did not show any significant effects on viable cell numbers of nondecidualized and 8-Br-cAMP-induced decidualized cells. Prolactin release from the 8-Br-cAMP-induced decidualized cells was not affected by EGF. However, EGF dose-dependently inhibited prolactin release from the stromal cells that were in the process of decidualization by co-stimulation with 8-Br-cAMP and EGF, though there was no significant change in viable cell numbers of the 8-Br-cAMP-stimulated decidualizing cells. Flow cytometric analysis revealed that 8-Br-cAMP enhanced EGF receptor expression on the endometrial stromal cells. These results indicate that endometrial EGF inhibits decidualization through autocrine/paracrine mechanisms.
尽管已有报道称子宫内膜上皮细胞和基质细胞可产生表皮生长因子（EGF），且子宫内膜基质细胞中存在EGF受体表达，但EGF对人子宫内膜基质细胞的影响尚未得到充分表征。我们使用人子宫内膜基质细胞的体外蜕膜化活性测定法，研究了EGF对子宫内膜细胞活力、8-溴环磷腺苷（8-Br-cAMP）诱导的基质蜕膜化以及蜕膜化子宫内膜基质细胞催乳素分泌的影响。EGF对未蜕膜化和8-Br-cAMP诱导的蜕膜化细胞的活细胞数量均未显示出任何显著影响。8-Br-cAMP诱导的蜕膜化细胞释放催乳素不受EGF影响。然而，尽管8-Br-cAMP刺激的蜕膜化细胞的活细胞数量没有显著变化，但EGF通过与8-Br-cAMP共同刺激，以剂量依赖的方式抑制正在蜕膜化的基质细胞释放催乳素。流式细胞术分析显示，8-Br-cAMP增强了子宫内膜基质细胞上EGF受体的表达。这些结果表明，子宫内膜EGF通过自分泌/旁分泌机制抑制蜕膜化。
