Tanaka T, Sakamoto T, Miyama M, Ogita S, Umesaki N
Department of Obstetrics and Gynecology, Wakayama Medical College, 811-1 Kimi-idera, Wakayama 641-8509, Japan.
Gynecol Endocrinol. 2001 Aug;15(4):272-8.
High expression of interleukin-11 (IL-11) and the IL-11 receptor alpha chain in developing decidual cells in mice has been reported, and mice lacking IL-11 receptor expression have been reported to show impaired implantation owing to defective decidualization. However, the direct effect of IL-11 on endometrial stromal cells has not been studied. In this study, we examined the direct effects of IL-11 on normal human endometrial stromal cells using an in vitro decidualization assay system. IL-11 enhanced cell viability and prolactin secretion of 8-Br-cAMP-induced decidualized cells but not of non-stimulated stromal cells. IL-11 dose-dependently enhanced the viability of stromal cells co-stimulated with 8-Br-cAMP and IL-11 without any significant effect on prolactin secretion from the cells. The extracellular matrix did not affect the effect of IL-11 on the viability of 8-Br-cAMP-stimulated stromal cells. These results indicate that IL-11 enhances the viability of 8-Br-cAMP-stimulated stromal cells and of decidualized stromal cells, and that the cell survival signals generated by IL-11 are independent of those generated by the extracellular matrix. IL-11 produced locally in decidual tissues may enhance the viability of decidualized stromal cells and possibly protect against cell damage during embryo implantation and trophoblastic invasion.
据报道，白细胞介素-11（IL-11）及其α链受体在小鼠蜕膜细胞发育过程中高表达，且据报道，缺乏IL-11受体表达的小鼠因蜕膜化缺陷而导致着床受损。然而，IL-11对子宫内膜基质细胞的直接作用尚未得到研究。在本研究中，我们使用体外蜕膜化检测系统研究了IL-11对正常人类子宫内膜基质细胞的直接作用。IL-11可增强8-溴环磷腺苷（8-Br-cAMP）诱导的蜕膜化细胞的细胞活力和催乳素分泌，但对未受刺激的基质细胞无此作用。IL-11呈剂量依赖性地增强了与8-Br-cAMP和IL-11共同刺激的基质细胞的活力，而对这些细胞的催乳素分泌无显著影响。细胞外基质不影响IL-11对8-Br-cAMP刺激的基质细胞活力的作用。这些结果表明，IL-11可增强8-Br-cAMP刺激的基质细胞和蜕膜化基质细胞的活力，且IL-11产生的细胞存活信号独立于细胞外基质产生的信号。蜕膜组织局部产生的IL-11可能增强蜕膜化基质细胞的活力，并可能在胚胎着床和滋养层侵袭过程中防止细胞损伤。
