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[中国黑腹果蝇分离畸变因子的频率分布及品系建立]

[The frequency distribution and establishment of fruit fly strain of segregation distorter in Drosophila melanogaster in China].

作者信息

Hao L, Gu Z L, Dai Z H

机构信息

College of Life Sciences, Peking University, Beijing 100871, China.

出版信息

Yi Chuan Xue Bao. 2000;27(4):298-303.

Abstract

Segregation Distorter (SD) is a meiotic drive system of natural occurrence. Heterozygous SD/SD+ males transmit the SD chromosome in vast excess over the normal homolog. SD chromosomes have been recovered at low frequency (1%-5%) from almost every population that has been screened for them in many places of the world. To examine whether there is SD system in natural populations of Drosophila melanogaster in China, we surveyed a few populations of D. melanogaster in Beijing and Qingdao respectively. The results suggested that SD is also found in every population examined at frequency of 1%-5%. On the basis of learning distribution of SD in China, we established a fruit fly stoch of SD from wild population of D. melanogaster in Beianhe district of Beijing. Furthermore, instead of using traditional genetic hybridization, we used molecular approach, PCR, to examine the distribution of SD chromosomes, which has been proved a very effective, quick and convenient method.

摘要

分离畸变因子(SD)是一种自然发生的减数分裂驱动系统。杂合的SD/SD+雄性个体传递SD染色体的频率远远超过正常同源染色体。在世界许多地方对几乎每个进行过筛查的种群中,都以低频率(1%-5%)发现了SD染色体。为了研究中国黑腹果蝇自然种群中是否存在SD系统,我们分别对北京和青岛的一些黑腹果蝇种群进行了调查。结果表明,在所检测的每个种群中都发现了SD,频率为1%-5%。基于了解SD在中国的分布情况,我们从北京北安河地区黑腹果蝇的野生种群中建立了一个SD果蝇品系。此外,我们没有使用传统的遗传杂交方法,而是采用分子方法——聚合酶链反应(PCR)来检测SD染色体的分布,这已被证明是一种非常有效、快速且便捷的方法。

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