从血浆蛋白中去除乙醇的冻干替代方法。II. 白蛋白的凝胶过滤

Alternatives to freeze-drying for the removal of ethanol from plasma proteins. II. Gel filtration of albumin.

作者信息

Dickson A J, Smith J K

出版信息

Vox Sang. 1975;28(2):90-100. doi: 10.1111/j.1423-0410.1975.tb02747.x.

DOI:10.1111/j.1423-0410.1975.tb02747.x

Abstract

Removal of ethanol from highly concentrated solutions of human albumin (Cohn fraction V) by gel filtration on Sephadex G-25 is hindered by the contraction of the gel in ethanolic solution, by incomplete retardation of ethanol compared with other low MW solutes, and by restricted diffusion of ethanol from the albumin zone. Despite these obstacles, the hourly capacity of such gel filtration columns, for approximately 100-fold reduction of ethanol concentration, may exceed 0.06 kg albumin per litre of column volume. The gel can be used safely at 5 degrees C for several years. The ethanol content of the final product is higher than that achieved by vacuum distillation, and it may be desirable to operate the two techniques sequentially.

摘要

通过葡聚糖凝胶G-25上的凝胶过滤从高浓度人白蛋白（科恩第五组分）溶液中去除乙醇受到以下因素的阻碍：乙醇溶液中凝胶的收缩、与其他低分子量溶质相比乙醇的不完全阻滞以及乙醇从白蛋白区域的扩散受限。尽管存在这些障碍，这种凝胶过滤柱每小时将乙醇浓度降低约100倍的处理能力可能超过每升柱体积0.06千克白蛋白。该凝胶可在5摄氏度安全使用数年。最终产品的乙醇含量高于真空蒸馏法获得的含量，可能需要依次采用这两种技术。