利用白细胞对胱氨酸病进行生化诊断。

Biochemical diagnosis of cystinosis using leukocytes.

作者信息

Willcox P, Patrick A D

出版信息

Acta Paediatr Scand. 1975 Jan;64(1):132-4. doi: 10.1111/j.1651-2227.1975.tb04390.x.

DOI:10.1111/j.1651-2227.1975.tb04390.x

Abstract

A simple method for the biochemical diagnosis of cystinosis involves incubating leukocytes from a small volume of blood with 35S-cystine. Non-protein 35S-labelled compounds are then extracted from the leucocytes and separated by thin-layer chromatography. The excessive incorporation of 35S-cystine into the increased cystine pool of cystine cells is easily detected in autoradiographs of the chromatograms.

摘要

一种用于胱氨酸病生化诊断的简单方法，是将少量血液中的白细胞与35S-胱氨酸一起孵育。然后从白细胞中提取非蛋白质35S标记的化合物，并通过薄层色谱法进行分离。在色谱图的放射自显影片中，很容易检测到35S-胱氨酸过度掺入到胱氨酸病细胞增加的胱氨酸池中。