一种用于分析35S-胱氨酸的高效液相色谱法：应用于胱氨酸病的诊断。
A high performance liquid chromatography method for the analysis of 35S-cystine: application to the diagnosis of cystinosis.
作者信息
Hall N A, Young E P
机构信息
Department of Clinical Biochemistry, Institute of Child Health, London UK.
出版信息
Clin Chim Acta. 1989 Sep 15;184(1):1-5. doi: 10.1016/0009-8981(89)90251-9.
An HPLC method for the measurement of radioactively labelled cystine is described. This method has been applied to studies of the uptake and retention of 35S-cystine by cultured cells. Radioactive cystine was measured, as a proportion of the non-protein labelled products in cultured cells incubated with medium containing 35S-cystine. Cells from healthy individuals contained less than 7% cystine whereas cells from cases of cystinosis contained at least 19% cystine. The method has been applied to the prenatal diagnosis of cystinosis. The use of flow radioactivity detection provides the advantages of rapid diagnosis and quantitation of metabolites.
描述了一种用于测量放射性标记胱氨酸的高效液相色谱法。该方法已应用于研究培养细胞对35S-胱氨酸的摄取和保留。测量放射性胱氨酸，作为在含有35S-胱氨酸的培养基中孵育的培养细胞中非蛋白质标记产物的一部分。健康个体的细胞含有的胱氨酸少于7%，而胱氨酸病患者的细胞至少含有19%的胱氨酸。该方法已应用于胱氨酸病的产前诊断。流动放射性检测的使用提供了快速诊断和定量代谢物的优点。
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