Pardo A G, Obertello M, Forchiassin F
Laboratorio de Micología Experimental, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina.
Rev Argent Microbiol. 2000 Oct-Dec;32(4):190-5.
The ability to produce cellulose and xylan degrading enzymes by different strains of Thecotheus pelletieri, in liquid synthetic media with cellulose and xylan as inducers, was compared. All the strains tested were able to grow and produce cellulases and xylanases, being the strain BAFC 2077 the best producer. Several cultural conditions were analysed in order to optimise enzyme production by strain 2077. Shaking cultures gave higher yields of cellulases and xylanases compared with stationary ones. Asparagine at 0.75 g N/L was the best nitrogen source in promoting enzyme production. The influence of different surfactants on enzyme production was studied. Tween 80 exhibited no effect on growth and enzyme production, whereas Tween 20 and Triton X-100 were inhibitory. By means of studies of variation of cellulose/xylan ratio in the culture medium we determined that cellulose and xylan induced cellulase and xylanase synthesis, being the specific substrates the most effective. The inducible behavior of cellulases and xylanases in T. pelletieri was determined by means of inhibition of protein synthesis by cycloheximide and ethidium bromide. Moreover, we found that glucose as well as xylose repressed cellulase and xylanase synthesis in T. pelletieri.
比较了不同菌株的佩氏梭囊菌（Thecotheus pelletieri）在以纤维素和木聚糖为诱导剂的液体合成培养基中产生纤维素降解酶和木聚糖降解酶的能力。所有测试菌株都能够生长并产生纤维素酶和木聚糖酶，其中菌株BAFC 2077是最佳生产者。分析了几种培养条件以优化2077菌株的酶产量。与静置培养相比，振荡培养产生的纤维素酶和木聚糖酶产量更高。0.75 g N/L的天冬酰胺是促进酶产生的最佳氮源。研究了不同表面活性剂对酶产生的影响。吐温80对生长和酶产生没有影响，而吐温20和曲拉通X-100具有抑制作用。通过研究培养基中纤维素/木聚糖比例的变化，我们确定纤维素和木聚糖诱导纤维素酶和木聚糖酶的合成，特定底物是最有效的。通过用环己酰亚胺和溴化乙锭抑制蛋白质合成来确定佩氏梭囊菌中纤维素酶和木聚糖酶的可诱导行为。此外，我们发现葡萄糖以及木糖会抑制佩氏梭囊菌中纤维素酶和木聚糖酶的合成。
