Sivori A S, Mercuri O A, Forchiassin F
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1996 Jan-Mar;28(1):9-15.
Growth and cellulase-xylanase enzyme systems production by Ascobolus gamundii were evaluated in synthetic media with or without natural inducers. The basal level of enzyme complexes was determined using glucose as carbon source. With crystalline cellulose, the amount of endoglucanase and beta-glucosidase was 2-4 times the basal, and the level of endoxylanase and beta-xylosidase was 3 times higher than that observed with glucose. When using xylan as carbon source, endoxylanase and endoglucanase were induced. The higher production of the enzyme systems was observed during the log-phase growth of A. gamundii with a mixture of cellulose and xylan as carbon source. The generalized induction of both enzyme complexes could be due to evolutive adaptation to the substrate (herbivore dung) in which cellulose and hemicellulose are associated to conform the plants cell-walls.
在添加或不添加天然诱导剂的合成培养基中，评估了加蒙地盘菌（Ascobolus gamundii）的生长及纤维素酶 - 木聚糖酶系统的产生。以葡萄糖作为碳源测定酶复合物的基础水平。使用结晶纤维素时，内切葡聚糖酶和β - 葡萄糖苷酶的量是基础水平的2 - 4倍，内切木聚糖酶和β - 木糖苷酶的水平比使用葡萄糖时观察到的高3倍。当使用木聚糖作为碳源时，内切木聚糖酶和内切葡聚糖酶被诱导。在加蒙地盘菌以纤维素和木聚糖混合物作为碳源的对数生长期，观察到酶系统的产量更高。两种酶复合物的普遍诱导可能是由于进化上对底物（食草动物粪便）的适应，其中纤维素和半纤维素结合形成植物细胞壁。
