Carnevale E M, Squires E L, Maclellan L J, Alvarenga M A, Scott T J
Animal Reproduction and Biotechnology Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523-1683, USA.
J Am Vet Med Assoc. 2001 Jan 1;218(1):87-91, 37. doi: 10.2460/javma.2001.218.87.
In some mares with lesions of the reproductive tract, embryo collection and survival rates are low, or collection of embryos is not feasible. For these mares, oocyte transfer has been proposed as a method to induce pregnancies. In this report, a method for oocyte transfer in mares and results of oocyte transfer performed over 2 breeding seasons, using mares with long histories of subfertility and various reproductive lesions, are described. Human chorionic gonadotropin or an implant containing a gonadotropin-releasing hormone analog was used to initiate follicular and oocyte maturation. Oocytes were collected by means of transvaginal ultrasound-guided follicular aspiration. Following follicular aspiration, cumulus oocyte complexes were evaluated for cumulus expansion and signs of atresia; immature oocytes were cultured in vitro to allow maturation. The recipient's ovary and uterine tube (oviduct) were exposed through a flank laparotomy with the horse standing, and the oocyte was slowly deposited within the oviduct. Oocyte transfer was attempted in 38 mares between 9 and 30 years old during 2 successive breeding seasons. All mares had a history of reproductive failure while in breeding and embryo transfer programs. Twenty pregnancies were induced. Fourteen of the pregnant mares delivered live foals. Results suggest that oocyte transfer can be a successful method for inducing pregnancy in subfertile mares in a commercial setting.
在一些患有生殖道病变的母马中，胚胎采集率和存活率较低，或者无法采集到胚胎。对于这些母马，卵母细胞移植已被提议作为一种诱导怀孕的方法。在本报告中，描述了一种母马卵母细胞移植方法以及在两个繁殖季节中对患有长期不育和各种生殖病变的母马进行卵母细胞移植的结果。使用人绒毛膜促性腺激素或含有促性腺激素释放激素类似物的植入物来启动卵泡和卵母细胞的成熟。通过经阴道超声引导下卵泡抽吸术采集卵母细胞。卵泡抽吸后，评估卵丘卵母细胞复合体的卵丘扩展和闭锁迹象；未成熟卵母细胞在体外培养以使其成熟。在马站立的情况下，通过侧腹剖腹术暴露受体的卵巢和输卵管，然后将卵母细胞缓慢注入输卵管内。在连续两个繁殖季节中，对38匹9至30岁的母马尝试进行了卵母细胞移植。所有母马在繁殖和胚胎移植计划中都有繁殖失败的历史。诱导了20次怀孕。其中14匹怀孕母马产下了活驹。结果表明，在商业环境中，卵母细胞移植可以成为诱导不育母马怀孕的一种成功方法。
