Carnevale Elaine M, Maclellan Lisa J, Coutinho da Silva Marco A, Squires Edward L
Animal Reproduction and Biotechnology laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
J Am Vet Med Assoc. 2003 Jan 1;222(1):60-2, 36. doi: 10.2460/javma.2003.222.60.
After euthanasia, ovaries were removed from 5 horses and shipped to a laboratory where 46 oocytes were collected. The oocytes were cultured for 24 to 30 hours, and 36 oocytes were transferred to 10 recipient mares via flank laparotomies. Recipient mares were inseminated with semen from various stallions. Sixteen days after transfer, 4 of the recipients were pregnant with at least 1 embryonic vesicle. Embryonic death occurred in 3 recipients, whereas a healthy live foal was born from 1 recipient. Ovaries from valuable mares can be a source of viable oocytes after death of the mare. For shipping to a laboratory, fluctuations in temperature should be minimized and the ovaries should not be chilled.
对5匹马实施安乐死后，取出卵巢并运至实验室，从中采集到46枚卵母细胞。将这些卵母细胞培养24至30小时，然后通过侧腹剖腹术将36枚卵母细胞移植到10匹受体母马体内。受体母马用来自不同种公马的精液进行授精。移植16天后，4匹受体母马怀有至少1个胚泡。3匹受体母马发生胚胎死亡，而1匹受体母马产下1匹健康活驹。珍贵母马死后，其卵巢可成为有活力卵母细胞的来源。运输至实验室时，应尽量减少温度波动，且卵巢不应受冻。
