Russo J, Furmanski P, Rich M A
Am J Anat. 1975 Feb;142(2):221-31. doi: 10.1002/aja.1001420207.
The ultrastructure of normal human mammary cells cultured from post-weaning breast fluids is described. Cells from confluent monolayers in two week old cultures were studied. The epithelial nature of these cells was established by the demonstration of a well developed system of cell-to-cell interdigitation and numerous desmosomes. These cells also share with breast epithelial cells in vivo, polarity, with blunt short microvilli on the apical surface and an oriented arrangement of organelles in the basal and apical portions of the cells. The Golgi apparatus, which is the most highly developed organelle, is localized in the apical pole and contains substantial quantities of secretory material in the cisternae and vesicles. A variegated palisade of finely granular material mixed with tonofilaments is seen in the basal portion of the cells; many of these tonofilaments end in the terminal web of the desmosomes. The regular occurrence of these cells in breast fluids during the terminal phases of lactation suggests that their separation is a part of normal breast involution.
描述了从断奶后母乳中培养的正常人乳腺细胞的超微结构。研究了两周龄培养物中汇合单层的细胞。通过细胞间指状交错发育良好的系统和大量桥粒的证明，确定了这些细胞的上皮性质。这些细胞在体内也与乳腺上皮细胞具有极性，顶端表面有钝短微绒毛，细胞基部和顶端部分的细胞器呈定向排列。高尔基体是发育最完善的细胞器，位于顶端极，其扁平囊和小泡中含有大量分泌物质。在细胞基部可见由细颗粒物质与张力丝混合而成的斑驳栅栏状结构；许多这些张力丝终止于桥粒的终末网。在泌乳末期母乳中经常出现这些细胞，表明它们的分离是正常乳腺退化的一部分。
