Uzzau S, Lu R, Wang W, Fiore C, Fasano A
Division of Pediatric Gastroenterology, Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.
FEMS Microbiol Lett. 2001 Jan 1;194(1):1-5. doi: 10.1111/j.1574-6968.2001.tb09437.x.
In the present study, we report the preliminary characterization of the epithelial cell receptor for Vibrio cholerae zonula occludens toxin (Zot). Zot receptor was purified by ligand-affinity chromatography. Analysis of affinity-purified preparations by polyacrylamide gel electrophoresis revealed a protein of ca. 66 kDa. Partial N-terminal sequence obtained from purified murine and human Zot receptor revealed homology between the two proteins and with human alpha-1-chimaerin. Zot protein domain(s) involved in receptor binding were also analyzed by constructing several in frame deletion derivatives of a recombinant fusion Zot protein tagged with maltose binding protein. Our results suggest that Zot binding to its cellular membrane receptor requires a sequence that spans between amino acids 118 and 299.
在本研究中，我们报告了霍乱弧菌小带闭合毒素（Zot）上皮细胞受体的初步特征。通过配体亲和层析法纯化Zot受体。用聚丙烯酰胺凝胶电泳分析亲和纯化的制剂，显示出一种约66 kDa的蛋白质。从纯化的小鼠和人Zot受体获得的部分N端序列揭示了这两种蛋白质之间以及与人α-1-嵌合蛋白之间的同源性。还通过构建几种带有麦芽糖结合蛋白标签的重组融合Zot蛋白的读框内缺失衍生物，分析了参与受体结合的Zot蛋白结构域。我们的结果表明，Zot与其细胞膜受体的结合需要一个跨越氨基酸118至299之间的序列。
