Chowdhury M A, Hill R T, Colwell R R
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore 21202.
FEMS Microbiol Lett. 1994 Jun 15;119(3):377-80. doi: 10.1111/j.1574-6968.1994.tb06916.x.
The presence of the zonula occludens toxin (ZOT) gene, which encodes an enterotoxin produced by serotype O1 strains of the pathogenic bacterium, Vibrio cholerae, in addition to cholera toxin, was investigated in selected strains of V. mimicus and the new pandemic V. cholerae non-O1 serotype O139. The zot gene was detected by polymerase chain reaction (PCR) amplification, using sets of primers based on the sequence of the V. cholerae O1 zot sequence. PCR amplification of genomic DNAs of both cholera toxin gene (ctx) positive and ctx- strains of V. mimicus detected the presence of zot gene. An AccI-EcoRV V. cholerae zot gene fragment designed to overlap PCR products was used as a probe. Southern hybridization studies confirmed that the PCR fragments from V. mimicus and V. cholerae O139 were strongly homologous to the V. cholerae O1 zot gene. The zot gene was found with 3 of 5 strains of V. mimicus of which only one strain harbored the ctx gene. The presence of a zot gene in ctx- toxigenic V. mimicus indicates a possible role of ZOT in the toxigenicity of this species. We conclude that, in addition to ctx, V. mimicus and V. cholerae O139 have the potential to produce ZOT.
除霍乱毒素外，还对所选的拟态弧菌菌株和新型大流行的霍乱弧菌非O1血清型O139菌株中是否存在小带闭合毒素（ZOT）基因进行了研究，该基因编码由致病性细菌霍乱弧菌O1血清型菌株产生的一种肠毒素。使用基于霍乱弧菌O1型zot序列设计的引物对，通过聚合酶链反应（PCR）扩增来检测zot基因。对拟态弧菌霍乱毒素基因（ctx）阳性和ctx阴性菌株的基因组DNA进行PCR扩增，均检测到zot基因的存在。将设计为与PCR产物重叠的AccI-EcoRV霍乱弧菌zot基因片段用作探针。Southern杂交研究证实，来自拟态弧菌和霍乱弧菌O139的PCR片段与霍乱弧菌O1型zot基因高度同源。在5株拟态弧菌中有3株检测到zot基因，其中只有1株携带ctx基因。ctx阴性产毒拟态弧菌中存在zot基因，表明ZOT在该菌种的产毒性中可能发挥作用。我们得出结论，除ctx外，拟态弧菌和霍乱弧菌O139都有产生ZOT的潜力。
Zotero 插件