Elmore L W, Domson K, Moore J R, Kornstein M, Burks R T
Department of Pathology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Va 23298, USA.
Arch Pathol Lab Med. 2001 Jan;125(1):146-51. doi: 10.5858/2001-125-0146-EOCKCI.
The proto-oncogene c-kit encodes a tyrosine kinase receptor (CD117) with a molecular weight of 145 kd. Previous studies, predominantly utilizing immunohistochemistry, have led to contradictory findings regarding the expression of CD117 in the endometrium. To help resolve this issue, we analyzed a series of benign and malignant endometrial tissues using both immunohistochemistry and Western blot analysis.
To examine the expression of CD117 in benign and malignant human endometrial tissues.
The expression of CD117 in 35 benign endometrial tissues (7 hyperplastic, 14 proliferative, 14 secretory) and 10 endometrioid carcinomas was investigated by immunohistochemistry (clone K45 monoclonal antibody). Immunoprecipitation (clone K69 monoclonal antibody) followed by Western blotting (clone K45 monoclonal antibody and clone 1.D9.3D6 monoclonal antibody) was performed to confirm CD117 expression.
Fifty-seven percent of the hyperplasias, 93% of proliferative endometria, and 79% of secretory endometria immunostained positively for CD117. In benign endometria, epithelial staining tended to be more intense in the hyperplastic and proliferative endometria as compared to the secretory endometria, whereas endometrial stromal cells were not immunoreactive. Of the 10 frozen endometrial tissues analyzed by immunohistochemistry, 4 of 9 endometrioid carcinomas and a single case of an endometrioid polyp developing in association with a carcinoma expressed CD117. Immunoprecipitation followed by Western blot analysis confirmed expression of full-length CD117 in an endometrial polyp and carcinoma, and revealed a correlation between levels of immunoprecipitated CD117 and immunohistochemical staining intensity.
Benign and malignant endometrial tissues express CD117. Our data suggest (a) a possible relationship between estrogen and CD117 expression in benign endometrium and (b) potential involvement of this growth factor receptor in endometrial carcinogenesis.
原癌基因c-kit编码一种分子量为145kd的酪氨酸激酶受体（CD117）。以往的研究主要采用免疫组织化学方法，对于CD117在子宫内膜中的表达得出了相互矛盾的结果。为了帮助解决这个问题，我们使用免疫组织化学和蛋白质印迹分析对一系列良性和恶性子宫内膜组织进行了分析。
检测CD117在人良性和恶性子宫内膜组织中的表达。
采用免疫组织化学（克隆K45单克隆抗体）检测35例良性子宫内膜组织（7例增生期、14例增殖期、14例分泌期）和10例子宫内膜样癌中CD117的表达。采用免疫沉淀（克隆K69单克隆抗体）后进行蛋白质印迹分析（克隆K45单克隆抗体和克隆1.D9.3D6单克隆抗体）以证实CD117的表达。
57%的增生期内膜、93%的增殖期内膜和79%的分泌期内膜CD117免疫染色呈阳性。在良性子宫内膜中，与分泌期内膜相比，增生期和增殖期内膜上皮染色往往更强，而子宫内膜间质细胞无免疫反应性。在通过免疫组织化学分析的10例冷冻子宫内膜组织中，9例子宫内膜样癌中的4例以及1例与癌相关的子宫内膜样息肉表达CD117。免疫沉淀后进行蛋白质印迹分析证实了子宫内膜息肉和癌中全长CD117的表达，并揭示了免疫沉淀的CD117水平与免疫组织化学染色强度之间的相关性。
良性和恶性子宫内膜组织均表达CD117。我们的数据表明：（a）雌激素与良性子宫内膜中CD117表达之间可能存在关系；（b）这种生长因子受体可能参与子宫内膜癌的发生。
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