Grauer J N, Patel T C, Erulkar J S, Troiano N W, Panjabi M M, Friedlaender G E
Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut 06520-8071, USA.
Spine (Phila Pa 1976). 2001 Jan 15;26(2):127-33. doi: 10.1097/00007632-200101150-00004.
An established rabbit intertransverse process lumbar fusion model was used to evaluate osteogenic protein (OP)-1 as a potential graft substitute.
To determine whether OP-1 is effective in producing intertransverse process lumbar fusion in a rabbit model.
Autogenous iliac crest bone is the gold standard in grafting material for inducing intertransverse process fusion. However, bone graft substitutes are being considered as supplementary or alternative means to achieve such fusion with less morbidity. Relatively little research has been undertaken to investigate the efficacy of OP-1 in this role.
Single-level intertransverse process lumbar fusions were performed at L5-L6 of 31 New Zealand White rabbits. These were divided into three study groups: autograft, carrier alone, and carrier with OP-1. The animals were killed 5 weeks after surgery. Resultant fusion masses were evaluated by manual palpation, radiography, biomechanical multidirectional flexibility testing, and histology.
Seven rabbits (23%) were excluded because of complications. Of the remaining 24 rabbits, 5 (63%) of the 8 in the autograft group had fusion detected by manual palpation, none (0%) of the 8 in the carrier-alone group had fusion, and all 8 (100%) in the OP-1 group had fusion. Radiographs were 55% sensitive and 92% specific for determining fusion. Biomechanical testing results correlated well with those of manual palpation. Histologically, autograft specimens were predominantly fibrocartilage, OP-1 specimens were predominantly maturing bone, and carrier-alone specimens did not show significant bone formation.
OP-1 was found to reliably induce solid intertransverse process fusion in a rabbit model at 5 weeks.
采用已建立的兔腰椎横突间融合模型来评估成骨蛋白（OP）-1作为一种潜在的移植替代物。
确定OP-1在兔模型中诱导腰椎横突间融合是否有效。
自体髂骨是诱导腰椎横突间融合的移植材料的金标准。然而，骨移植替代物正被视为实现这种融合且发病率较低的补充或替代方法。关于OP-1在这一作用方面的疗效的研究相对较少。
对31只新西兰白兔在L5-L6节段进行单节段腰椎横突间融合术。将这些兔子分为三个研究组：自体移植组、单纯载体组和含OP-1载体组。术后5周处死动物。通过手动触诊、X线摄影、生物力学多向柔韧性测试和组织学评估融合块。
7只兔子（23%）因并发症被排除。在其余24只兔子中，自体移植组8只中有5只（63%）通过手动触诊检测到融合，单纯载体组8只中无一例（0%）融合，OP-1组8只全部（100%）融合。X线摄影在确定融合方面的敏感性为55%，特异性为92%。生物力学测试结果与手动触诊结果相关性良好。组织学上，自体移植标本主要为纤维软骨，OP-1标本主要为成熟骨，单纯载体标本未显示明显的骨形成。
发现在兔模型中，OP-1在5周时能可靠地诱导坚实的腰椎横突间融合。
