[Identification of partially deuterated exometabolites during culture of tea fungi in heavy water].

Exometabolites of tea fungus during the cultivation on heavy water were studied by methods of high-resolution proton and deuterium exchange. Is was shown that, depending on the degree of exchange of proton for deuterium in the methyl group of acetate, three isotopomers exist: -CH3, -CH2D, and -CHD2. Ethanol is represented by six isotopomers: -CHD-CH3, -CD2-CH3, -CD2-CH2D, -CD2-CHD2, -CHD-CH2D, and -CHD-CHD2. Relative concentration of these isotopomers and the percent content of deuterium were determined. Based on these data, the kinetics of metabolism of the object cultivated on heavy water was analyzed. It was show that the efficiency of glucose utilization is the same as during cultivation on light water with the exception that the time of utilization is significantly greater. At the same time, the parameters of utilization of ethanol and its conversion into acetic acid in the life activity of the tea fungus in heavy and light water are very similar.