Kutyshenko V P, Iurkevich D I
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Biofizika. 2001 May-Jun;46(3):452-9.
High-resolution 1H-NMR experiments were performed on 2H/13C isotopically labelled metabolites of growing medusomycete culture. Some minor metabolites were identified and the degree of their deuterization was established. Some of these metabolites were shown to be present in isomeric forms. Our results demonstrated that glycerol was produced in the growth medium on the early stage of the system adaptation to the growth in D2O.
对正在生长的水母菌培养物的 2H/13C 同位素标记代谢物进行了高分辨率 1H-NMR 实验。鉴定出了一些次要代谢物，并确定了它们的氘化程度。结果表明，其中一些代谢物以异构体形式存在。我们的结果表明，在系统适应 D2O 中生长的早期阶段，甘油在生长培养基中产生。
