Syversen T L
Arch Environ Health. 1975 Mar;30(3):158-61. doi: 10.1080/00039896.1975.10666666.
Autopsy specimens of liver and kidneys from 40 patients have been analyzed for cadmium and zinc. Sephadex chromatography was performed on soluble extracts from these tissues. In samples from 19 patients a cadmium-binding protein was found. The mean cadmium content of kidney in this group was higher than the corresponding value for all 40 patients. A molecular weight of 10 to 12,000 was estimated for the cadmium-binding protein by Sephadex chromatography. Isoelectric focusing resolved two peaks with pl 4.8 and pl 6.0, the OD254/OD280ratio for both peaks being about 5. These data indicate that humans without known occupational exposure to cadmium do have a cadmium-binding protein similar to metallothionein in their liver and kidney.
对40例患者的肝脏和肾脏尸检标本进行了镉和锌分析。对这些组织的可溶性提取物进行了葡聚糖凝胶色谱分析。在19例患者的样本中发现了一种镉结合蛋白。该组患者肾脏中的镉平均含量高于所有40例患者的相应值。通过葡聚糖凝胶色谱法估计镉结合蛋白的分子量为10,000至12,000。等电聚焦分离出两个峰，其等电点分别为4.8和6.0，两个峰的OD254/OD280比值约为5。这些数据表明，没有已知职业性镉暴露的人在其肝脏和肾脏中确实有一种类似于金属硫蛋白的镉结合蛋白。
