日本慢生根瘤菌USDA 110合成低分子量形式的胞外多糖。
Synthesis of a low-molecular-weight form of exopolysaccharide by Bradyrhizobium japonicum USDA 110.
作者信息
Louch H A, Miller K J
机构信息
Intercollege Graduate Program in Genetics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
出版信息
Appl Environ Microbiol. 2001 Feb;67(2):1011-4. doi: 10.1128/AEM.67.2.1011-1014.2001.
Abstract
A novel extracellular low-molecular-weight polysaccharide was detected as a contaminant within extracellular cyclic beta-1,6-beta-1,3-glucan preparations from Bradyrhizobium japonicum USDA 110 cultures. Compositional analysis, methylation analysis, and nuclear magnetic resonance analysis revealed that this low-molecular-weight polysaccharide was composed of the same pentasaccharide repeating unit previously described for the high-molecular-weight form of the exopolysaccharide (EPS) synthesized by B. japonicum strains. Mass spectrometry analysis indicated that the size of this low-molecular-weight form of EPS was consistent with a dimeric form of the pentasaccharide repeating unit.
摘要
在日本慢生根瘤菌USDA 110培养物的细胞外环状β-1,6-β-1,3-葡聚糖制剂中检测到一种新型细胞外低分子量多糖作为污染物。组成分析、甲基化分析和核磁共振分析表明，这种低分子量多糖由与日本慢生根瘤菌菌株合成的胞外多糖（EPS）高分子量形式先前描述的相同五糖重复单元组成。质谱分析表明，这种低分子量形式的EPS大小与五糖重复单元的二聚体形式一致。
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