基于镍抗性的微型转座子：用于环境细菌基因操作的新工具。
Nickel-resistance-based minitransposons: new tools for genetic manipulation of environmental bacteria.
作者信息
Taghavi S, Delanghe H, Lodewyckx C, Mergeay M, van der Lelie D
机构信息
Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium.
出版信息
Appl Environ Microbiol. 2001 Feb;67(2):1015-9. doi: 10.1128/AEM.67.2.1015-1019.2001.
Abstract
The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha, beta, and gamma subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.
摘要
来自类真养产碱菌31A菌株的ncc和nre镍抗性决定因子被用于构建mini-Tn5转座子。在变形菌门的α、β和γ亚类成员中，观察到nre微型转座子具有广泛宿主的镍抗性表达，而ncc微型转座子仅在类真养产碱菌菌株中表达镍抗性。
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