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核苷酸切除修复复合物的结构：DNA在损伤识别前后均被UvrB包裹。

Architecture of nucleotide excision repair complexes: DNA is wrapped by UvrB before and after damage recognition.

作者信息

Verhoeven E E, Wyman C, Moolenaar G F, Hoeijmakers J H, Goosen N

机构信息

Laboratory of Molecular Genetics, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.

出版信息

EMBO J. 2001 Feb 1;20(3):601-11. doi: 10.1093/emboj/20.3.601.

DOI:10.1093/emboj/20.3.601
PMID:11157766
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC133479/
Abstract

Nucleotide excision repair (NER) is a major DNA repair mechanism that recognizes a broad range of DNA damages. In Escherichia coli, damage recognition in NER is accomplished by the UvrA and UvrB proteins. We have analysed the structural properties of the different protein-DNA complexes formed by UvrA, UvrB and (damaged) DNA using atomic force microscopy. Analysis of the UvrA(2)B complex in search of damage revealed the DNA to be wrapped around the UvrB protein, comprising a region of about seven helical turns. In the UvrB-DNA pre-incision complex the DNA is wrapped in a similar way and this DNA configuration is dependent on ATP binding. Based on these results, a role for DNA wrapping in damage recognition is proposed. Evidence is presented that DNA wrapping in the pre-incision complex also stimulates the rate of incision by UvrC.

摘要

核苷酸切除修复（NER）是一种主要的DNA修复机制，可识别多种DNA损伤。在大肠杆菌中，NER中的损伤识别是由UvrA和UvrB蛋白完成的。我们使用原子力显微镜分析了由UvrA、UvrB和（受损）DNA形成的不同蛋白质-DNA复合物的结构特性。对UvrA(2)B复合物进行损伤搜索分析发现，DNA缠绕在UvrB蛋白周围，形成约七个螺旋圈的区域。在UvrB-DNA预切割复合物中，DNA以类似方式缠绕，且这种DNA构型依赖于ATP结合。基于这些结果，提出了DNA缠绕在损伤识别中的作用。有证据表明，预切割复合物中的DNA缠绕也会刺激UvrC的切割速率。

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本文引用的文献

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