Wakamatsu Y, Ju B, Pristyaznhyuk I, Niwa K, Ladygina T, Kinoshita M, Araki K, Ozato K
Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1071-6. doi: 10.1073/pnas.98.3.1071.
Fertile and diploid nuclear transplants were successfully generated by using embryonic cells as donors in a small laboratory fish, medaka (Oryzias latipes). Embryonic cell nuclei from transgenic fish carrying the green fluorescent protein (GFP) gene were transplanted into unfertilized eggs enucleated by x-ray irradiation. In this study, 1 out of 588 eggs transplanted in the first experiment and 5 out of 298 eggs transplanted in the second experiment reached the adult stage. All of these nuclear transplants were fertile and diploid, and the natural and GFP markers of the donor nuclei were transmitted to the F(1) and F(2) offspring in a Mendelian fashion. This systematic study proves the feasibility of generating nuclear transplants by using embryonic cells from fish as donors, and it is supported by convincing evidence.
在小型实验鱼类青鳉（Oryzias latipes）中，通过使用胚胎细胞作为供体，成功培育出了可育的二倍体核移植鱼。将携带绿色荧光蛋白（GFP）基因的转基因鱼的胚胎细胞核移植到经X射线照射去核的未受精卵中。在本研究中，第一次实验移植的588枚卵中有1枚、第二次实验移植的298枚卵中有5枚发育至成年阶段。所有这些核移植鱼都是可育的二倍体，供体细胞核的天然标记和GFP标记以孟德尔方式传递给F(1)和F(2)代后代。这项系统性研究证明了利用鱼类胚胎细胞作为供体进行核移植的可行性，且有令人信服的证据支持。
