源自小型实验鱼类青鳉（Oryzias latipes）胚胎细胞的可育二倍体核移植体。
Fertile and diploid nuclear transplants derived from embryonic cells of a small laboratory fish, medaka (Oryzias latipes).
作者信息
Wakamatsu Y, Ju B, Pristyaznhyuk I, Niwa K, Ladygina T, Kinoshita M, Araki K, Ozato K
机构信息
Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
出版信息
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1071-6. doi: 10.1073/pnas.98.3.1071.
Abstract
Fertile and diploid nuclear transplants were successfully generated by using embryonic cells as donors in a small laboratory fish, medaka (Oryzias latipes). Embryonic cell nuclei from transgenic fish carrying the green fluorescent protein (GFP) gene were transplanted into unfertilized eggs enucleated by x-ray irradiation. In this study, 1 out of 588 eggs transplanted in the first experiment and 5 out of 298 eggs transplanted in the second experiment reached the adult stage. All of these nuclear transplants were fertile and diploid, and the natural and GFP markers of the donor nuclei were transmitted to the F(1) and F(2) offspring in a Mendelian fashion. This systematic study proves the feasibility of generating nuclear transplants by using embryonic cells from fish as donors, and it is supported by convincing evidence.
摘要
在小型实验鱼类青鳉（Oryzias latipes）中，通过使用胚胎细胞作为供体，成功培育出了可育的二倍体核移植鱼。将携带绿色荧光蛋白（GFP）基因的转基因鱼的胚胎细胞核移植到经X射线照射去核的未受精卵中。在本研究中，第一次实验移植的588枚卵中有1枚、第二次实验移植的298枚卵中有5枚发育至成年阶段。所有这些核移植鱼都是可育的二倍体，供体细胞核的天然标记和GFP标记以孟德尔方式传递给F(1)和F(2)代后代。这项系统性研究证明了利用鱼类胚胎细胞作为供体进行核移植的可行性，且有令人信服的证据支持。
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本文引用的文献
1
On the Inheritance of Color in a Fresh-Water Fish, APLOCHEILUS LATIPES Temmick and Schlegel, with Special Reference to Sex-Linked Inheritance.关于淡水鱼五线无须魮（Temmick和Schlegel）颜色的遗传，特别涉及伴性遗传
Genetics. 1921 Nov;6(6):554-73. doi: 10.1093/genetics/6.6.554.
2
Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.将囊胚细胞的活细胞核移植到去核的蛙卵中。
Proc Natl Acad Sci U S A. 1952 May;38(5):455-63. doi: 10.1073/pnas.38.5.455.
3
Expression of GFP in nuclear transplants generated by transplantation of embryonic cell nuclei from GFP-transgenic fish into nonenucleated eggs of medaka, Oryzias latipes.绿色荧光蛋白（GFP）在核移植中的表达情况，该核移植是通过将来自绿色荧光蛋白转基因鱼的胚胎细胞核移植到青鳉（Oryzias latipes）的非去核卵中产生的。
Cloning. 2000;2(1):23-34. doi: 10.1089/15204550050145102.
4
Activity of the medaka translation elongation factor 1alpha-A promoter examined using the GFP gene as a reporter.以绿色荧光蛋白（GFP）基因作为报告基因检测青鳉翻译延伸因子1α-A启动子的活性。
Dev Growth Differ. 2000 Oct;42(5):469-78. doi: 10.1046/j.1440-169x.2000.00530.x.
5
Introduction of a foreign gene into medakafish using the particle gun method.使用粒子枪方法将外源基因导入青鳉鱼。
J Exp Zool. 2000 Sep 1;287(4):285-93.
6
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells.通过培养的体细胞进行核移植生产基因靶向绵羊。
Nature. 2000 Jun 29;405(6790):1066-9. doi: 10.1038/35016604.
7
Cloning: pathways to a pluripotent future.
Science. 2000 Jun 9;288(5472):1775-80. doi: 10.1126/science.288.5472.1775.
8
Generation of mice from wild-type and targeted ES cells by nuclear cloning.通过核克隆从野生型和靶向胚胎干细胞生成小鼠。
Nat Genet. 2000 Feb;24(2):109-10. doi: 10.1038/72753.
9
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Dev Growth Differ. 1999 Apr;41(2):163-72. doi: 10.1046/j.1440-169x.1999.00423.x.
10
Cloned transgenic calves produced from nonquiescent fetal fibroblasts.由非静止胎儿成纤维细胞产生的克隆转基因小牛。
Science. 1998 May 22;280(5367):1256-8. doi: 10.1126/science.280.5367.1256.
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