Revoltella R, Bosman C, Bertolini L
Cancer Res. 1975 Apr;35(4):890-5.
A cloned suspension culture of mouse C1300 neuroblastoma cells bound, at 2degrees, sheep erythrocytes passively coated with nerve growth factor, with the formation of rosettes. When grown in tissue culture dishes to which they could attach, neuroblastoma cells rapidly transformed, within 48 hr emitting cytoplasmic processes some of which were several mm long. Most of the attached neuroblastoma cells formed rosettes. In contrast, normal mouse kidney cells or various murine tumor cell lines used as cell controls exhibited a poor capacity for binding nerve growth factor. Rosette formation was a specific reaction that could be prevented by pretreating cells with proteolytic enzymes, free nerve growth factor, or specific antibodies against neuroblastoma cell extracts.
小鼠C1300神经母细胞瘤细胞的克隆悬浮培养物在2℃时与被动包被神经生长因子的绵羊红细胞结合，形成玫瑰花结。当在它们能够附着的组织培养皿中生长时，神经母细胞瘤细胞迅速发生转化，在48小时内发出细胞质突起，其中一些长达数毫米。大多数附着的神经母细胞瘤细胞形成玫瑰花结。相比之下，用作细胞对照的正常小鼠肾细胞或各种鼠肿瘤细胞系结合神经生长因子的能力较差。玫瑰花结的形成是一种特异性反应，可用蛋白水解酶、游离神经生长因子或针对神经母细胞瘤细胞提取物的特异性抗体预处理细胞来阻止。
