Diamond L, Revoltella R, Bertolini L
Brain Res. 1976 Dec 24;118(3):453-9. doi: 10.1016/0006-8993(76)90312-7.
The expression of receptors for nerve growth factor (NGF) on the cell surface was assayed by rosette formation with ligand-coated sheep red blood cells (SRBC). Cell clones derived from the murine C1300 neuroblastoma and from hybrids between a neuroblastoma clone and L cell clones showed a wide variation in the capacity to form rosettes with NGF-coated SRBC. All the neuroblastoma, L cell and hybrid clones formed rosettes with phytohemagglutinin-coated SRBC and none formed rosettes with cytochrome c- or ferritin-coated SRBC or with SRBC not coated with ligand.
通过与配体包被的绵羊红细胞（SRBC）形成玫瑰花结来检测细胞表面神经生长因子（NGF）受体的表达。源自小鼠C1300神经母细胞瘤以及神经母细胞瘤克隆与L细胞克隆之间杂种的细胞克隆，在与NGF包被的SRBC形成玫瑰花结的能力上表现出很大差异。所有神经母细胞瘤、L细胞和杂种克隆都能与植物血凝素包被的SRBC形成玫瑰花结，而与细胞色素c或铁蛋白包被的SRBC或未包被配体的SRBC均不形成玫瑰花结。
