来自鱼腥藻Anabaena PCC 7119的重组铁摄取调节蛋白（Fur）与isiB及其自身启动子的克隆、过表达及相互作用
Cloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isiB and its own promoter.
作者信息
Bes M T, Hernández J A, Peleato M L, Fillat M F
机构信息
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna s/n, 50009, Saragossa, Spain.
出版信息
FEMS Microbiol Lett. 2001 Jan 15;194(2):187-92. doi: 10.1111/j.1574-6968.2001.tb09467.x.
A gene coding for a Fur (ferric uptake regulation) protein from the cyanobacterium Anabaena PCC 7119 has been cloned and overexpressed in Escherichia coli. DNA sequence analysis confirmed the presence of a 151-amino-acid open reading frame that showed homology with the Fur proteins reported for the unicellular cyanobacteria Synechococcus 7942 and Synechocystis PCC 6803. Two putative Fur-binding sites were detected in the promoter regions of the fur gene from Anabaena. Partially purified recombinant Fur binds to the flavodoxin promoter as well as its own promoter. This suggests that the Fur gene is autoregulated in Anabaena.
编码来自鱼腥藻Anabaena PCC 7119的铁摄取调节（Fur）蛋白的基因已被克隆并在大肠杆菌中过表达。DNA序列分析证实存在一个151个氨基酸的开放阅读框，该阅读框与单细胞蓝细菌聚球藻Synechococcus 7942和集胞藻Synechocystis PCC 6803中报道的Fur蛋白具有同源性。在鱼腥藻的fur基因启动子区域检测到两个假定的Fur结合位点。部分纯化的重组Fur与黄素氧还蛋白启动子及其自身启动子结合。这表明Fur基因在鱼腥藻中是自我调节的。
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