来自鱼腥藻PCC7120的三种fur同源物：探索相互的蛋白质-启动子识别
Three fur homologues from Anabaena sp. PCC7120: exploring reciprocal protein-promoter recognition.
作者信息
Hernández José A, López-Gomollón Sara, Bes M Teresa, Fillat María F, Peleato M Luisa
机构信息
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain.
出版信息
FEMS Microbiol Lett. 2004 Jul 15;236(2):275-82. doi: 10.1016/j.femsle.2004.05.050.
DNA sequence analysis of the Anabaena sp. PCC7120 genome confirmed the presence of three open reading frames (all1691, all2473 and alr0957) containing the histidine-rich region characteristic of the Fur family. The genes coding for the three Fur proteins were cloned and overexpressed in Escherichia coli. The overexpression products, called FurA, FurB and FurC are only distantly related. The ability of the three recombinant proteins to bind iron-boxes identified in the three fur promoter regions was tested by electrophoretical mobility shift assays. FurA binds the three fur promoters with increased affinity in presence of metal. FurB also binds the three fur promoters, and its affinity is increased with DTT. FurC does not bind to furA or furB promoter regions or to its own promoter. However, FurC affects the ability of FurB and FurA to bind their target promoters.
鱼腥藻属PCC7120基因组的DNA序列分析证实存在三个开放阅读框（all1691、all2473和alr0957），其含有铁调节蛋白（Fur）家族特有的富含组氨酸区域。编码这三种Fur蛋白的基因被克隆并在大肠杆菌中过表达。过表达产物分别称为FurA、FurB和FurC，它们之间的亲缘关系较远。通过电泳迁移率变动分析测试了这三种重组蛋白与在三个fur启动子区域中鉴定出的铁框结合的能力。FurA在金属存在下以增加的亲和力结合这三个fur启动子。FurB也结合这三个fur启动子，并且其亲和力在二硫苏糖醇（DTT）存在下增加。FurC不结合furA或furB启动子区域或其自身的启动子。然而，FurC影响FurB和FurA结合其靶启动子的能力。
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