Doherty H M, Adams D G
Department of Microbiology, University of Leeds, UK.
Gene. 1995 Sep 22;163(1):93-6. doi: 10.1016/0378-1119(95)00416-4.
Using degenerate oligodeoxyribonucleotide primers based on conserved regions of the cell-division protein FtsZ, a 220-bp fragment of DNA was amplified by the polymerase chain reaction from Anabaena PCC 7120 (Ana). This fragment, which showed significant homology with Escherichia coli ftsZ, was used as a probe to isolate a 15-kb genomic clone containing ftsZ from an Ana DNA library. Sequence analysis revealed an open reading frame (ORF) encoding a protein of 379 amino acids, with 49% identity with E. coli FtsZ. Upstream of Ana ftsZ is a small, unidentified ORF, transcribed in the same direction. An ORF lying downstream of the ftsZ coding region and transcribed in the opposite orientation, shows homology with bacterial glutathione synthetase-encoding genes. Single copies of ftsZ have been identified in Ana and two other cyanobacteria. Multiple transcripts hybridising to ftsZ were detected by Northern hybridisation.
基于细胞分裂蛋白FtsZ的保守区域使用简并寡脱氧核糖核苷酸引物，通过聚合酶链反应从鱼腥藻PCC 7120（Ana）中扩增出一段220 bp的DNA片段。该片段与大肠杆菌ftsZ具有显著同源性，被用作探针从Ana DNA文库中分离出一个包含ftsZ的15 kb基因组克隆。序列分析揭示了一个编码379个氨基酸的开放阅读框（ORF），与大肠杆菌FtsZ的同源性为49%。Ana ftsZ的上游是一个未鉴定的小ORF，转录方向相同。位于ftsZ编码区下游且转录方向相反的一个ORF与细菌谷胱甘肽合成酶编码基因具有同源性。在Ana和另外两种蓝细菌中已鉴定出ftsZ的单拷贝。通过Northern杂交检测到与ftsZ杂交的多个转录本。
