成人人类心脏微血管内皮细胞对人巨细胞病毒的非裂解性感染具有易感性。
Adult human heart microvascular endothelial cells are permissive for non-lytic infection by human cytomegalovirus.
作者信息
Ricotta D, Alessandri G, Pollara C, Fiorentini S, Favilli F, Tosetti M, Mantovani A, Grassi M, Garrafa E, Dei Cas L, Muneretto C, Caruso A
机构信息
Institute of Microbiology, University of Brescia, P.le Spedali Civili 1, 25123, Brescia, Italy.
出版信息
Cardiovasc Res. 2001 Feb 1;49(2):440-8. doi: 10.1016/s0008-6363(00)00258-3.
OBJECTIVE
Human cytomegalovirus (CMV) infection has been linked to chronic heart disease. The mechanism of CMV dissemination to the heart remains unknown. CMV antigens and nucleic acid sequences have been detected in endothelial cells (ECs) in vivo, and ECs are fully permissive hosts to CMV replication in vitro. This report examines the characteristics of CMV replication in primary cultures of human heart microvascular ECs (HHMECs).
METHODS
Capillary ECs were isolated from heart tissue biopsies of six patients at the time of heart surgery. HHMECs were infected with CMV and viral antigens were detected by immunofluorescence assay using monoclonal antibodies as specific reagents. Cytokine and chemokine release in the supernatant of sham- and CMV-infected cells was quantitated by ELISA. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to analyse expression of mRNA for adhesion molecules.
RESULTS
CMV was found to productively infect HHMECs without cytolytic effects. Infected cultures released high levels of pro-inflammatory chemokines and enhanced their adhesion molecule expression.
CONCLUSIONS
Our data provide new insights into the mechanism of CMV dissemination to the heart, signalling the need for further investigation of the pathogenetic role of this virus in cardiac disorders.
目的
人类巨细胞病毒（CMV）感染与慢性心脏病有关。CMV传播至心脏的机制尚不清楚。在体内已在内皮细胞（ECs）中检测到CMV抗原和核酸序列，并且ECs在体外是CMV复制的完全允许宿主。本报告研究了人心脏微血管内皮细胞（HHMECs）原代培养物中CMV复制的特征。
方法
在心脏手术时从6例患者的心脏组织活检中分离出毛细血管内皮细胞。HHMECs用CMV感染，并用单克隆抗体作为特异性试剂通过免疫荧光测定法检测病毒抗原。用ELISA定量假感染和CMV感染细胞上清液中细胞因子和趋化因子的释放。逆转录聚合酶链反应（RT-PCR）用于分析粘附分子mRNA的表达。
结果
发现CMV可有效感染HHMECs且无细胞溶解作用。受感染的培养物释放出高水平的促炎趋化因子并增强其粘附分子表达。
结论
我们的数据为CMV传播至心脏的机制提供了新的见解，表明需要进一步研究该病毒在心脏疾病中的致病作用。
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