Pedrón N, Giner J, Hicks J J, Rosado A
Fertil Steril. 1975 Apr;26(4):309-13. doi: 10.1016/s0015-0282(16)41049-6.
The glycolysis of spermatozoa from normal, asthenospermic, and oligoasthen ospermic men was studied using a respirometry technique to measure glucose utilization by the production of 14CO2 from glucose 14C (U-L). Lactate and pyruvate were measured by a spectrophotometric method using DNA as reference. Human spermatozoa preferred glucose to fructose as the glycolytic substrate when concentrations of these hexoses did not exceed normal concentrations in the blood. Spermatozoa from oligoasthenospermic men produced an average of 3.5 times more 14CO2 (345, 457 dpm/mg DNA/hour) than did spermatozoa from asthenospermic (88,837 dpm/mg DNA/hour) and normal men (96,595 dpm/mg DNA/hour). They also formed four times more lactate (9.63 mumoles/mg DNA/hour) than spermatozoa from normal men (2.33 mumoles/mg DNA/hour) and 6.4 times more pyruvate (2.90 mumoles/mg DNA/hour compared to 0.45 mumoles/mg DNA/hour). Spermatozoa from asthenospermic men formed amounts of lactate (3.01 mumoles/mg DNA/hour) and pyruvate (0.63 mumoles/mg DNA/hour) similar to those produced by spermatozoa from normal men.
采用呼吸测量技术，通过测量14C葡萄糖（U-L）产生的14CO2来测定葡萄糖利用率，对正常男性、弱精子症男性和少弱精子症男性的精子糖酵解进行了研究。以DNA为参比，采用分光光度法测定乳酸和丙酮酸。当这些己糖的浓度不超过血液中的正常浓度时，人类精子优先选择葡萄糖而非果糖作为糖酵解底物。少弱精子症男性的精子产生的14CO2平均比弱精子症男性（88,837 dpm/mg DNA/小时）和正常男性（96,595 dpm/mg DNA/小时）的精子多3.5倍（345,457 dpm/mg DNA/小时）。它们形成的乳酸（9.63微摩尔/mg DNA/小时）也比正常男性的精子（2.33微摩尔/mg DNA/小时）多四倍，丙酮酸（2.90微摩尔/mg DNA/小时，而正常男性为0.45微摩尔/mg DNA/小时）多6.4倍。弱精子症男性的精子形成的乳酸（3.01微摩尔/mg DNA/小时）和丙酮酸（0.63微摩尔/mg DNA/小时）量与正常男性的精子产生的量相似。
