Hunter S J, Thompson F J, Tetley L, Devaney E
Department of Veterinary Parasitology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.
Mol Biochem Parasitol. 2001 Jan 15;112(1):1-9. doi: 10.1016/s0166-6851(00)00339-x.
The temporal expression pattern of two genes, Bp-cdd and Bp-S3, was studied at defined points throughout the life cycle of Brugia pahangi. Both mRNAs were up-regulated to coincide with the transition of the L3 from the vector to the mammalian host. Bp-cdd was expressed almost exclusively in the post-infective (p.i.) L3 and L4 stages of the life cycle while Bp-S3 was also expressed in adult worms, but at a much lower level than in the larval stages. Immunogold labelling with an antiserum raised to the recombinant Bp-CDD localised the native antigen to the hypodermis in the p.i. L3 and L4. Specific labelling was not detected in the adult worm. The expression of both mRNAs could be triggered by exposure of the vector-derived L3 to a simple mammalian culture system. Analysis of the factors, which induced expression suggested that the temperature shift which accompanies the transition from mosquito to mammal was the most important cue for expression of both genes.
在马来丝虫整个生命周期的特定时间点，研究了两个基因Bp-cdd和Bp-S3的表达模式。两种mRNA均上调，与L3从媒介向哺乳动物宿主的转变同步。Bp-cdd几乎仅在生命周期的感染后（p.i.）L3和L4阶段表达，而Bp-S3在成虫中也有表达，但水平远低于幼虫阶段。用针对重组Bp-CDD产生的抗血清进行免疫金标记，将天然抗原定位到p.i. L3和L4的皮下组织。在成虫中未检测到特异性标记。将媒介来源的L3暴露于简单的哺乳动物培养系统可触发两种mRNA的表达。对诱导表达的因素分析表明，从蚊子到哺乳动物转变时伴随的温度变化是这两个基因表达的最重要线索。
