Marnett L J, Reed G A
Biochemistry. 1979 Jul 10;18(14):2923-9. doi: 10.1021/bi00581a001.
The arachidonic acid dependent oxidation of benzo[a]pyrene to a mixture of 3,6-, 1,6-, and 6,12-quinones has been studied by using enzyme preparations from sheep seminal vesicles. Maximal oxidation is observed at 100 microM benzo[a]pyrene and 150 microM arachidonic acid. The arachidonic acid dependent oxidation is peroxidatic and utilizes prostaglandin G2 (PGG2), generated in situ from arachidonate, as the hydroperoxide substrate. 15-Hydroperoxy-5,8,11,13-eicosatetraenoic acid is equivalent to PGG2 as a hydroperoxide substrate, but hydrogen peroxide, cumene hydroperoxide, and tert-butyl hydroperoxide are much poorer substrates. Arachidonic acid dependent benzo[a]pyrene oxidation by microsomal and solubilized enzyme preparations is markedly.
利用绵羊精囊的酶制剂，研究了花生四烯酸依赖的苯并[a]芘氧化生成3,6 -、1,6 -和6,12 -醌混合物的过程。在100微摩尔苯并[a]芘和150微摩尔花生四烯酸时观察到最大氧化。花生四烯酸依赖的氧化是过氧化物酶性的，并利用由花生四烯酸原位生成的前列腺素G2（PGG2）作为氢过氧化物底物。15 - 氢过氧 - 5,8,11,13 - 二十碳四烯酸作为氢过氧化物底物与PGG2相当，但过氧化氢、异丙苯过氧化氢和叔丁基过氧化氢是差得多的底物。微粒体和可溶酶制剂对花生四烯酸依赖的苯并[a]芘氧化作用明显。
