表达相同抗原特异性受体的CD8(+)和CD8(-) T细胞在抗原识别和细胞溶解活性方面的差异。
Differences in antigen recognition and cytolytic activity of CD8(+) and CD8(-) T cells that express the same antigen-specific receptor.
作者信息
Cho B K, Lian K C, Lee P, Brunmark A, McKinley C, Chen J, Kranz D M, Eisen H N
机构信息
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1723-7. doi: 10.1073/pnas.98.4.1723.
Abstract
CD8(+) and CD8(-) T cell lines expressing the same antigen-specific receptor [the 2C T cell receptor (TCR)] were compared for ability to bind soluble peptide-MHC and to lyse target cells. The 2C TCR on CD8(-) cells bound a syngeneic MHC (K(b+))-peptide complex 10-100 times less well than the same TCR on CD8(+) cells, and the CD8(-) 2C cells lysed target cells presenting this complex very poorly. Surprisingly, however, the CD8(-) cells differed little from CD8(+) cells in ability to bind an allogeneic MHC (L(d+))-peptide complex and to lyse target cells presenting this complex. The CD8(+)/CD8(-) difference provided an opportunity to estimate how long TCR engagements with peptide-MHC have to persist to initiate the cytolytic T cell response.
摘要
对表达相同抗原特异性受体[2C T细胞受体（TCR）]的CD8(+)和CD8(-) T细胞系进行了比较，以研究它们结合可溶性肽-MHC以及裂解靶细胞的能力。CD8(-)细胞上的2C TCR与同基因MHC（K(b+)）-肽复合物的结合能力比CD8(+)细胞上相同的TCR低10至100倍，并且CD8(-) 2C细胞对呈递这种复合物的靶细胞的裂解能力非常差。然而，令人惊讶的是，CD8(-)细胞在结合异基因MHC（L(d+)）-肽复合物以及裂解呈递这种复合物的靶细胞的能力方面与CD8(+)细胞几乎没有差异。CD8(+)/CD8(-)的差异为估计TCR与肽-MHC的结合需要持续多长时间才能启动细胞毒性T细胞反应提供了一个机会。
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本文引用的文献
1
Dependence of lymphopenia-induced T cell proliferation on the abundance of peptide/ MHC epitopes and strength of their interaction with T cell receptors.淋巴细胞减少诱导的T细胞增殖对肽/MHC表位丰度及其与T细胞受体相互作用强度的依赖性。
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1728-33. doi: 10.1073/pnas.98.4.1728.
2
Breaking the affinity ceiling for antibodies and T cell receptors.突破抗体和T细胞受体的亲和力上限。
Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10679-81. doi: 10.1073/pnas.97.20.10679.
3
Homeostasis-stimulated proliferation drives naive T cells to differentiate directly into memory T cells.稳态刺激增殖促使初始T细胞直接分化为记忆T细胞。
J Exp Med. 2000 Aug 21;192(4):549-56. doi: 10.1084/jem.192.4.549.
4
In vitro evolution of a T cell receptor with high affinity for peptide/MHC.对肽/主要组织相容性复合体具有高亲和力的T细胞受体的体外进化
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5387-92. doi: 10.1073/pnas.080078297.
5
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J Exp Med. 2000 Apr 17;191(8):1355-64. doi: 10.1084/jem.191.8.1355.
6
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J Exp Med. 2000 Jan 17;191(2):335-46. doi: 10.1084/jem.191.2.335.
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