使用最少的抗体进行有效的流式细胞术细胞表型分析。
Effective flow cytometric phenotyping of cells using minimal amounts of antibody.
机构信息
Department of Oncology, University of Wisconsin School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53706, USA.
出版信息
Biotechniques. 2012 Jul;53(1):57-60. doi: 10.2144/0000113854.
Abstract
Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.
摘要
在这里，我们介绍了一种改良的抗体染色方法，该方法用于流式细胞术时所需的抗体用量比传统方法减少了 80%。我们用该方法检测了在小鼠和大鼠淋巴细胞以及小鼠乳腺上皮细胞中高、中、低水平表达的抗原。我们获得了可重复且准确的结果，能够检测多达七个参数，包括激活诱导的增殖、细胞周期分析以及细胞表面和细胞内抗原的表型分析。
相似文献
1
Effective flow cytometric phenotyping of cells using minimal amounts of antibody.使用最少的抗体进行有效的流式细胞术细胞表型分析。
Biotechniques. 2012 Jul;53(1):57-60. doi: 10.2144/0000113854.
2
Clonal Analysis of Mouse Mammary Luminal Epithelial Cell Transplants.小鼠乳腺管腔上皮细胞移植的克隆分析
Stem Cells Dev. 2019 Mar 1;28(5):353-355. doi: 10.1089/scd.2018.0158. Epub 2019 Jan 31.
3
Classification of the Immune Composition in the Tumor Infiltrate.肿瘤浸润中免疫成分的分类
Methods Mol Biol. 2019;1979:305-315. doi: 10.1007/978-1-4939-9240-9_19.
4
OMIP-023: 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes.OMIP - 023：用于人类外周血白细胞深度表型分析的10色13抗体组合
Cytometry A. 2014 Sep;85(9):781-4. doi: 10.1002/cyto.a.22505. Epub 2014 Aug 4.
5
Isolation of mouse pancreatic ductal progenitor cells expressing CD133 and c-Met by flow cytometric cell sorting.通过流式细胞术细胞分选分离表达CD133和c-Met的小鼠胰腺导管祖细胞。
Gastroenterology. 2007 Feb;132(2):720-32. doi: 10.1053/j.gastro.2006.11.027. Epub 2006 Nov 18.
6
7
Multiple immunofluorescence analysis of cells using flow cytometry.
Methods Mol Biol. 1998;80:337-46. doi: 10.1007/978-1-59259-257-9_34.
8
Analysis of immunolabeled cells by atomic force microscopy, optical microscopy, and flow cytometry.通过原子力显微镜、光学显微镜和流式细胞术对免疫标记细胞进行分析。
J Struct Biol. 1994 Jan-Feb;112(1):32-40. doi: 10.1006/jsbi.1994.1004.
9
A cytofluorometric method for the simultaneous detection of both intracellular and surface antigens of apoptotic peripheral lymphocytes.一种用于同时检测凋亡外周淋巴细胞细胞内和表面抗原的细胞荧光测定方法。
J Immunol Methods. 1998 Aug 1;217(1-2):11-26. doi: 10.1016/s0022-1759(98)00060-x.
10
Multicolor flow cytometric analysis in SIV-infected rhesus macaque.对感染猴免疫缺陷病毒（SIV）的恒河猴进行多色流式细胞术分析。
Methods Cell Biol. 2004;75:535-57. doi: 10.1016/s0091-679x(04)75022-0.
引用本文的文献
1
Clobetasol propionate, a Nrf-2 inhibitor, sensitizes human lung cancer cells to radiation-induced killing via mitochondrial ROS-dependent ferroptosis.丙酸氯倍他索，一种 Nrf-2 抑制剂，通过线粒体 ROS 依赖性铁死亡使肺癌细胞对辐射诱导的杀伤敏感。
Acta Pharmacol Sin. 2024 Jul;45(7):1506-1519. doi: 10.1038/s41401-024-01233-8. Epub 2024 Mar 13.
2
Malabaricone C, a constituent of spice , exhibits anti-inflammatory effects via modulation of cellular redox.荜茇宁 C，一种香料成分，通过调节细胞氧化还原发挥抗炎作用。
J Biosci. 2023;48(2). doi: 10.1007/s12038-023-00329-3.
3
A magnetic micropore chip for rapid (<1 hour) unbiased circulating tumor cell isolation and in situ RNA analysis.一种用于快速（<1 小时）无偏循环肿瘤细胞分离和原位 RNA 分析的磁性微孔芯片。
Lab Chip. 2017 Sep 12;17(18):3086-3096. doi: 10.1039/c7lc00703e.
4
Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood.利用从血液中筛选出的循环白血病细胞，通过微流控技术检测急性髓系白血病患者的微小残留病
Analyst. 2016 Jan 21;141(2):640-51. doi: 10.1039/c5an01836f.
5
The gene desert mammary carcinoma susceptibility locus Mcs1a regulates Nr2f1 modifying mammary epithelial cell differentiation and proliferation.基因荒漠型乳腺癌易感位点 Mcs1a 调控 Nr2f1 从而改变乳腺上皮细胞的分化和增殖。
PLoS Genet. 2013 Jun;9(6):e1003549. doi: 10.1371/journal.pgen.1003549. Epub 2013 Jun 13.
本文引用的文献
1
Quantification of epithelial cell differentiation in mammary glands and carcinomas from DMBA- and MNU-exposed rats.DMBA 和 MNU 暴露大鼠乳腺和癌组织中上皮细胞分化的定量研究。
PLoS One. 2011;6(10):e26145. doi: 10.1371/journal.pone.0026145. Epub 2011 Oct 12.
2
The non-protein coding breast cancer susceptibility locus Mcs5a acts in a non-mammary cell-autonomous fashion through the immune system and modulates T-cell homeostasis and functions.非蛋白编码乳腺癌易感基因 Mcs5a 通过免疫系统以非乳腺细胞自主的方式发挥作用，并调节 T 细胞的稳态和功能。
Breast Cancer Res. 2011 Aug 16;13(4):R81. doi: 10.1186/bcr2933.
3
Keeping abreast of the mammary epithelial hierarchy and breast tumorigenesis.紧跟乳腺上皮层级结构与乳腺肿瘤发生的研究进展。
Genes Dev. 2009 Nov 15;23(22):2563-77. doi: 10.1101/gad.1849509.
4
Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach.用于精确测量T细胞亚群和细胞因子应答的九色流式细胞术。第一部分：基于经验方法的检测方案设计
Cytometry A. 2008 May;73(5):400-10. doi: 10.1002/cyto.a.20555.
5
Interleukin-7 receptor expression: intelligent design.白细胞介素-7受体表达：智能设计。
Nat Rev Immunol. 2007 Feb;7(2):144-54. doi: 10.1038/nri2023.
6
Generation of a functional mammary gland from a single stem cell.从单个干细胞生成功能性乳腺。
Nature. 2006 Jan 5;439(7072):84-8. doi: 10.1038/nature04372.
7
Purification and unique properties of mammary epithelial stem cells.乳腺上皮干细胞的纯化及其独特性质
Nature. 2006 Feb 23;439(7079):993-7. doi: 10.1038/nature04496. Epub 2006 Jan 4.
8
Human gammadelta T cells express a higher TCR/CD3 complex density than alphabeta T cells.人类γδ T细胞表达的TCR/CD3复合物密度高于αβ T细胞。
Clin Immunol. 2001 Mar;98(3):358-63. doi: 10.1006/clim.2000.4978.
9
Differences in antigen recognition and cytolytic activity of CD8(+) and CD8(-) T cells that express the same antigen-specific receptor.表达相同抗原特异性受体的CD8(+)和CD8(-) T细胞在抗原识别和细胞溶解活性方面的差异。
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1723-7. doi: 10.1073/pnas.98.4.1723.
10
Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation.细胞上CD4抗原密度的测定：抗体价、亲和力、克隆及偶联的作用
Cytometry. 1998 Oct 1;33(2):197-205. doi: 10.1002/(sici)1097-0320(19981001)33:2<197::aid-cyto14>3.0.co;2-p.
Zotero 插件