用于淋病奈瑟菌分离的选择性培养基的评估。
Assessment of a selective medium for the isolation of Neisseria gonorrhoeae.
作者信息
Taylor E, Phillips I
出版信息
Br J Vener Dis. 1979 Jun;55(3):183-5. doi: 10.1136/sti.55.3.183.
Abstract
Specimens of urethral pus from 312 men with gonorrhoea were diluted and inoculated on to non-selective and selective media, the latter containing vancomycin, colistin, nystatin, and trimethoprim. Although three (1%) isolates of Neisseria gonorrhoeae were inhibited by trimethoprim 4 mg/1 and 10 by vancomycin 2 mg/1, only two failed to grow on a selective medium from large inocula but eight strains sensitive to vancomycin failed to grow on a selective medium from a light inoculum. These few failures do not appear to negate the value of the selective medium.
摘要
对312名淋病男性患者的尿道脓性标本进行稀释，并接种于非选择性培养基和选择性培养基上，后者含有万古霉素、黏菌素、制霉菌素和甲氧苄啶。尽管有3株（1%）淋病奈瑟菌分离株被4mg/1的甲氧苄啶抑制，10株被2mg/1的万古霉素抑制，但只有2株在大量接种时未能在选择性培养基上生长，而8株对万古霉素敏感的菌株在少量接种时未能在选择性培养基上生长。这些少数失败情况似乎并未否定选择性培养基的价值。
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本文引用的文献
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VCN--inhibited strains of Neisseria gonorrhoeae.
HSMHA Health Rep. 1971 Nov;86(11):990-2.
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Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory.淋病奈瑟菌的万古霉素敏感菌株。诊断实验室面临的一个问题。
Br J Vener Dis. 1973 Oct;49(5):452-3. doi: 10.1136/sti.49.5.452.
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In-vitro activity of twelve antibacterial agents against Neisseria gonorrhoeae.十二种抗菌剂对淋病奈瑟菌的体外活性
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4
Diagnosis of gonorrhoea by culture on a selective medium containing vancomycin, colistin, nystatin and trimethoprim (VCNT). A comparison with Gram-staining and immunofluorescence.采用含万古霉素、黏菌素、制霉菌素和甲氧苄啶（VCNT）的选择性培养基进行淋病培养诊断。与革兰氏染色和免疫荧光法的比较。
Br J Vener Dis. 1972 Aug;48(4):287-92. doi: 10.1136/sti.48.4.287.
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