Kondo M, Kondo M, Udono T, Jin W Z, Jin W Z, Funakoshi M, Shimizu K, Itoh M, Herath C B, Watanabe G, Watanabe G, Groome N P, Taya K, Taya K
Novartis Animal Health, Tokyo 105-6134, Japan.
J Endocrinol. 2001 Feb;168(2):257-62. doi: 10.1677/joe.0.1680257.
Plasma concentrations of inhibin A and inhibin B during pregnancy and early lactation in chimpanzees were determined by enzyme-linked immunosorbent assay (ELISA). Plasma samples were taken from five pregnant chimpanzees at 6-9, 10, 20 and 25 weeks of pregnancy, and following parturition. Throughout pregnancy and the early postpartum period, circulating inhibin A and inhibin B concentrations remained low, at similar levels to those during the normal menstrual cycle in chimpanzees. Concentrations of inhibin A in the placental homogenate were high enough to be measured by the ELISA and by bioassay, whereas circulating inhibin bioactivities in late pregnancy were too low to be measured. Plasma concentrations of FSH remained low with no significant changes throughout pregnancy and the postpartum period. Plasma concentrations of oestradiol-17beta and progesterone at 25 weeks of pregnancy were much higher than normal menstrual cycle levels. It was concluded that in chimpanzees the levels of circulating inhibin A and inhibin B remained low throughout pregnancy and the early postpartum period, and that the concentrations of bioactive dimeric inhibin did not increase towards the end of pregnancy. The suppression of circulating FSH levels during pregnancy is suggested to be controlled by steroid hormones that increased significantly in late pregnancy, and the present findings further suggest that the secretory pattern and role of inhibin during pregnancy in chimpanzees may be different from that in human and other primates.
采用酶联免疫吸附测定法（ELISA）测定了黑猩猩孕期和哺乳期早期血浆中抑制素A和抑制素B的浓度。采集了5只怀孕黑猩猩在孕期6 - 9周、10周、20周和25周以及分娩后的血浆样本。在整个孕期和产后早期，循环中的抑制素A和抑制素B浓度一直较低，与黑猩猩正常月经周期时的水平相似。胎盘匀浆中抑制素A的浓度高到足以通过ELISA和生物测定法进行测量，而孕晚期循环中的抑制素生物活性过低无法测量。整个孕期和产后期间，血浆促卵泡生成素（FSH）浓度一直较低，没有显著变化。怀孕25周时血浆雌二醇 - 17β和孕酮的浓度远高于正常月经周期水平。得出的结论是，在黑猩猩中，整个孕期和产后早期循环中的抑制素A和抑制素B水平一直较低，并且生物活性二聚体抑制素的浓度在孕期结束时并未升高。孕期循环中FSH水平的抑制被认为是由孕晚期显著升高的甾体激素控制的，目前的研究结果进一步表明，黑猩猩孕期抑制素的分泌模式和作用可能与人类及其他灵长类动物不同。
