de Micco P
C R Seances Acad Sci D. 1979 Apr 23;288(15):1211-4.
A method is described to demonstrate and measure the cell-fusion in vitro induced by viruses. This technique has been established using Sendai virus. It has been used to study the fusing ability of the Scrapie agent which is responsible of a slowly progressing spongiform encephalopathy in Mice. In Vero cells, the Scrapie agent induces a fusion which appears slowly and remains moderate. This test can help to detect the human spongiform encephalopathies.
本文描述了一种用于证明和测量病毒在体外诱导细胞融合的方法。该技术是利用仙台病毒建立的。它已被用于研究羊瘙痒病病原体的融合能力,该病原体可导致小鼠发生缓慢进展的海绵状脑病。在非洲绿猴肾细胞(Vero细胞)中,羊瘙痒病病原体诱导的融合出现缓慢且程度适中。该试验有助于检测人类海绵状脑病。