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[小鼠瘙痒病病原体在体外诱导的细胞融合]

[Cellular fusion induced in vitro by the mouse scrapie agent].

作者信息

de Micco P

出版信息

C R Seances Acad Sci D. 1979 Apr 23;288(15):1211-4.

PMID:111844
Abstract

A method is described to demonstrate and measure the cell-fusion in vitro induced by viruses. This technique has been established using Sendai virus. It has been used to study the fusing ability of the Scrapie agent which is responsible of a slowly progressing spongiform encephalopathy in Mice. In Vero cells, the Scrapie agent induces a fusion which appears slowly and remains moderate. This test can help to detect the human spongiform encephalopathies.

摘要

本文描述了一种用于证明和测量病毒在体外诱导细胞融合的方法。该技术是利用仙台病毒建立的。它已被用于研究羊瘙痒病病原体的融合能力,该病原体可导致小鼠发生缓慢进展的海绵状脑病。在非洲绿猴肾细胞(Vero细胞)中,羊瘙痒病病原体诱导的融合出现缓慢且程度适中。该试验有助于检测人类海绵状脑病。

相似文献

1
[Cellular fusion induced in vitro by the mouse scrapie agent].[小鼠瘙痒病病原体在体外诱导的细胞融合]
C R Seances Acad Sci D. 1979 Apr 23;288(15):1211-4.
2
[Cell fusion induced in vitro by spongiform encephalopathy agents. Value of a diagnostic test].[海绵状脑病病原体在体外诱导的细胞融合。一种诊断测试的价值]
Rev Neurol (Paris). 1980;136(1):71-2.
3
[In vitro fusion ability of agents causing slow diseases of the central nervous system].[引起中枢神经系统慢病毒疾病的病原体的体外融合能力]
C R Seances Soc Biol Fil. 1979;173(1):117-21.
4
Prion encephalopathies of animals and humans.动物和人类的朊病毒脑病
Dev Biol Stand. 1993;80:31-44.
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Prion protein and the scrapie agent: in vitro studies in infected neuroblastoma cells.朊病毒蛋白与羊瘙痒病病原体:对感染的神经母细胞瘤细胞的体外研究
Infect Agents Dis. 1994 Apr-Jun;3(2-3):54-8.
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[The biology of prions, a response to the enigma of spongiform encephalopathies of sheep and humans].[朊病毒生物学,对绵羊和人类海绵状脑病之谜的回应]
Bull Acad Natl Med. 1990 Dec;174(9):1353-7; discussion 1357-9.
7
Comparative sequence analysis, in vitro expression and biosynthesis of mouse PrP.
Prog Clin Biol Res. 1989;317:619-36.
8
Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein.感染性绵羊瘙痒病病原体在表达羊朊病毒蛋白的异源上皮细胞中的体外增殖。
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4055-9. doi: 10.1073/pnas.061337998. Epub 2001 Mar 20.
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[Molecular pathogenesis of spongiform encephalopathies].
Verh Dtsch Ges Pathol. 1997;81:35-47.
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[Trials of in vitro propagation of the scrapie agent in mouse nerve cells].
C R Seances Acad Sci III. 1981 Nov 2;293(8):413-7.

引用本文的文献

1
Comparison of cell-fusing activity of brain suspensions from patients with Creutzfeldt-Jakob disease and other degenerative neurological diseases.克雅氏病及其他退行性神经疾病患者脑悬液细胞融合活性的比较
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5365-7. doi: 10.1073/pnas.76.10.5365.