Kulakov Iu K, Zheludkov M M, Tolmacheva T A, Alekseeva N V, Kovalev Iu N, Skavronskaia A G, Gintsburg A L, Mikhaĭlova Iu P, Kalmykov V V, Shumilov K V
N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow.
Mol Gen Mikrobiol Virusol. 2000(4):7-12.
Ten strains isolated from sick dogs in 1998 in St. Petersburg were studied by traditional and molecular biological methods of Brucella identification. PCR study confirmed that the isolated cultures were Brucellae, and comparative study of the traditional phenotypical characteristics and protein and antigenic composition allowed referring all the isolated strains to B. canis. Traditional identification showed similarity of 7 strains with the reference B. canis strain RM6/66, and 3 strains were similar to B. canis Mex 51 strain. These results confirmed the division of B. canis into two biovars. Polyacrylamide gel electrophoresis with sodium dodecyl sulfate demonstrated the identity of protein profiles of 10 strains isolated from dogs to the reference B. canis RM6/66 strain. Immunoblotting analysis with S- and R-specific rabbit antisera also demonstrated the identity of antigens binding IgG antibodies in the strains isolated from dogs to the reference B. canis RM6/66 strain.
1998年从圣彼得堡患病犬中分离出的10株菌株，采用布鲁氏菌传统鉴定方法和分子生物学方法进行研究。PCR研究证实分离出的培养物为布鲁氏菌，对传统表型特征以及蛋白质和抗原组成的比较研究表明，所有分离菌株均属于犬种布鲁氏菌。传统鉴定显示，7株菌株与参考犬种布鲁氏菌菌株RM6/66相似，3株菌株与犬种布鲁氏菌Mex 51菌株相似。这些结果证实犬种布鲁氏菌可分为两个生物变种。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，从犬中分离出的10株菌株的蛋白质图谱与参考犬种布鲁氏菌RM6/66菌株相同。用S和R特异性兔抗血清进行的免疫印迹分析也表明，从犬中分离出的菌株中与IgG抗体结合的抗原与参考犬种布鲁氏菌RM6/66菌株相同。
