Leal-Klevezas D S, López-Merino A, Martínez-Soriano J P
Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, N.L.
Arch Med Res. 1995 Autumn;26(3):263-7.
Brucellosis is an important zoonotic disease caused by several species of the genus Brucella. The most reliable diagnostic tests are based on microbiological analysis, bacterial growth, and biochemical reactions which are cumbersome and represent a risk of infection for technicians performing them. Recently, safe molecular genetic techniques have been incorporated in studies regarding taxonomy and evolution of Brucella. The polymerase chain reaction (PCR) was used here to analyze a duplicated gene (omp 2) encoding an outer membrane protein in members of the genus Brucella. The developed procedure detects each of the five species herein tested and their biovars: B. abortus, B. suis, B. melitensis, B. canis and B. ovis. It also permits the detection of an approximately 115 bp deletion in the omp 2a gene, consistently found in five different strains of B. abortus biovar 1: vaccine strain S19, rough mutant RB51, reference strains 544 and A99, and a strain isolated from naturally infected bovines.
布鲁氏菌病是由布鲁氏菌属的几个物种引起的一种重要的人畜共患病。最可靠的诊断测试基于微生物分析、细菌生长和生化反应，这些方法既繁琐，又对进行检测的技术人员存在感染风险。最近，安全的分子遗传学技术已被应用于布鲁氏菌的分类学和进化研究中。本文使用聚合酶链反应（PCR）来分析布鲁氏菌属成员中编码外膜蛋白的一个重复基因（omp 2）。所开发的方法能够检测本文测试的五个物种及其生物变种：流产布鲁氏菌、猪布鲁氏菌、羊布鲁氏菌、犬布鲁氏菌和绵羊布鲁氏菌。它还能检测到omp 2a基因中大约115 bp的缺失，该缺失在流产布鲁氏菌生物变种1的五个不同菌株中均能持续检测到：疫苗株S19、粗糙突变株RB51、参考菌株544和A99，以及从自然感染牛中分离出的一个菌株。
