[被孢霉产生花生四烯酸的研究]
[Studies on arachidonic acid production by Mortierella].
作者信息
Bao S, Zhu F, Lin W, Yao R
机构信息
Dept. of Bioengineering, South China University of Technology, Guangzhou 510641.
出版信息
Wei Sheng Wu Xue Bao. 1997 Oct;37(5):374-7.
The effects of the incubation temperature, initial pH of the medium, carbon source and nitrogen source on the production of arachidonic acid by Mortierella sp. M10 were studied. Thought orthogonal experiments, the optimum culture medium was obtained (g/L): glucose, 100; yeast extract, 10; KNO3, 4.0; KH2PO4, 2.0; CaCl2.2H2O, 0.1; MgSO4.7H2O, 0.5; FeCl3.6H2O, 0.015; ZnSO4.7H2O, 0.0075; CuSO4.5H2O, 0.0005. Under the optimum culture conditions, the dry cell weight and arachidonic acid was 33.51 g/L and 0.827 g/L, respectively. The flask culture process was analysed.
研究了培养温度、培养基初始pH值、碳源和氮源对被孢霉属M10产花生四烯酸的影响。通过正交试验，获得了最佳培养基（g/L）：葡萄糖100；酵母提取物10；KNO₃ 4.0；KH₂PO₄ 2.0；CaCl₂·2H₂O 0.1；MgSO₄·7H₂O 0.5；FeCl₃·6H₂O 0.015；ZnSO₄·7H₂O 0.0075；CuSO₄·5H₂O 0.0005。在最佳培养条件下，干细胞重量和花生四烯酸分别为33.51 g/L和0.827 g/L。对摇瓶培养过程进行了分析。
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