Jin Ming-Jie, Huang He, Xiao Ai-Hua, Gao Zhen, Liu Xin, Peng Chao
College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
Bioprocess Biosyst Eng. 2009 Jan;32(1):117-22. doi: 10.1007/s00449-008-0229-1. Epub 2008 May 15.
Traditional mycelium aging technology was improved to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1. Filtration step was skipped and additional carbon and nitrogen sources were fed during aging. The levels of the significant factors (time, temperature, ethanol, and KNO(3)) affecting ARA production during improved aging process were also optimized by applying response surface methodology (RSM), and the maximum ARA yield of 19.02 g/l was achieved in a 5 l fermentor at 5.6 days, temperature 13.7 degrees C, ethanol 42.44 g/l, and KNO(3) 2.62 g/l. This yield was 1.55 times higher than that of traditional aging technology. The improved mycelium aging technology is considered to be a useful strategy for enhancing ARA production.
对传统的菌丝体老化技术进行了改进，以提高高山被孢霉ME-1的花生四烯酸（ARA）产量。在老化过程中跳过了过滤步骤，并添加了额外的碳源和氮源。还通过应用响应面法（RSM）对改进老化过程中影响ARA产量的显著因素（时间、温度、乙醇和KNO₃）水平进行了优化，在5升发酵罐中，于5.6天、温度13.7℃、乙醇42.44克/升和KNO₃ 2.62克/升的条件下，获得了19.02克/升的最大ARA产量。该产量比传统老化技术高出1.55倍。改进的菌丝体老化技术被认为是提高ARA产量的一种有效策略。
